ArticlePLoS biology2023
Regulation of sleep by cholinergic neurons located outside the central brain in Drosophila.
Article in PLoS biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 41 citations in OpenAlex.
- Advances in Targeting Central Cholinergic Dysfunction for Neurodegenerative Diseases: From Pharmacotherapy to Neuromodulation.CNS neuroscience & therapeutics · 2026Review
- Context-dependent arousal via wake-promoting pontine neurons in the Drosophila melanogaster fan-shaped body.Sleep · 2026Article
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- Octopamine regulates neural circuits in the mushroom body and central complex, influencing sleep and arousal.iScience · 2026Article
- Sleep rescues age-associated loss of glial engulfment.PLoS genetics · 2026Article
- Sleep regulation inFrontiers in neuroscience · 2026Review
- 25 years ofFly · 2025Review
- The Glutamate-gated Chloride Channel Facilitates Sleep by Enhancing the Excitability of Two Pairs of Neurons in the Ventral Nerve Cord of Drosophila.Neuroscience bulletin · 2025Article
- A familial natural short sleep mutation iniScience · 2025Article
- Sleep drive, not total sleep amount, increases seizure risk.Nature communications · 2025Article
- Clock-dependent regulation of a homeostatic sleep center maintains daytime sleep and evening activity.Current biology : CB · 2025Article
- Insight into molecular and neural mechanisms of general anesthesia from the invertebrate modelZoological research · 2025Review
- Role of d-serine in intestinal ROS accumulation after sleep deprivation.Science advances · 2025Article
- Cell-specific tools for understanding behavior.eLife · 2025Article
- Refining the sleep circuits one neuron at a time.PLoS biology · 2025Article
- FlyVISTA, an integrated machine learning platform for deep phenotyping of sleep inScience advances · 2025Article
- The dorsal fan-shaped body is a neurochemically heterogeneous sleep-regulating center in Drosophila.PLoS biology · 2025Article
- Intermittent Vibration Induces Sleep via an Allatostatin A-GABA Signaling Pathway and Provides Broad Benefits in Alzheimer's Disease Models.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Four SpsP neurons are an integrating sleep regulation hub inScience advances · 2024Article
- Stem cell-specific ecdysone signaling regulates the development of dorsal fan-shaped body neurons and sleep homeostasis.Current biology : CB · 2024Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sleep is a complex and plastic behavior regulated by multiple brain regions and influenced by numerous internal and external stimuli. Thus, to fully uncover the function(s) of sleep, cellular resolution of sleep-regulating neurons needs to be achieved. Doing so will help to unequivocally assign a role or function to a given neuron or group of neurons in sleep behavior. In the Drosophila brain, neurons projecting to the dorsal fan-shaped body (dFB) have emerged as a key sleep-regulating area. To dissect the contribution of individual dFB neurons to sleep, we undertook an intersectional Split-GAL4 genetic screen focusing on cells contained within the 23E10-GAL4 driver, the most widely used tool to manipulate dFB neurons. In this study, we demonstrate that 23E10-GAL4 expresses in neurons outside the dFB and in the fly equivalent of the spinal cord, the ventral nerve cord (VNC). Furthermore, we show that 2 VNC cholinergic neurons strongly contribute to the sleep-promoting capacity of the 23E10-GAL4 driver under baseline conditions. However, in contrast to other 23E10-GAL4 neurons, silencing these VNC cells does not block sleep homeostasis. Thus, our data demonstrate that the 23E10-GAL4 driver contains at least 2 different types of sleep-regulating neurons controlling distinct aspects of sleep behavior.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.