ArticleIntegrative and comparative biology2023
A Fast And Versatile Method for Simultaneous HCR, Immunohistochemistry And Edu Labeling (SHInE).
Article in Integrative and comparative biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Faster and smaller: Heterochronic shift in gene expression leads to larval onset of spermatogenesis and miniaturized males.iScience · 2026Article
- Molecular evidence for early deuterostome origins of ovarian cell types and neuroendocrine control of reproduction.Nature communications · 2026Article
- In Situ Hybridization Chain Reaction and Immunohistochemical Labeling of the Octopamine Production Pathway in the Central Nervous System of Lymnaea stagnalis.The Journal of comparative neurology · 2026Article
- activeDISCO enables large-scale exploration of human brain anatomy by light sheet microscopy.Communications biology · 2026Article
- Photoreceptor control ofProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Light-modulated stem cells in the camera-type eye of an annelid model for adult brain plasticity.Nature communications · 2025Article
- Next-generation hybridization chain reaction tools with enhanced sensitivities to detect challenging targets.bioRxiv : the preprint server for biology · 2025Article
- 'Iterative Bleaching Extends Multiplexity' facilitates simultaneous identification of all major retinal cell types.Journal of cell science · 2024Article
- Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model.Nature communications · 2024Article
- Expression of novel androgen receptors in three GnRH neuron subtypes in the cichlid brain.Journal of neuroendocrinology · 2024Article
- Environmental and molecular control of tissue-specific ionocyte differentiation in zebrafish.Development (Cambridge, England) · 2024Article
- Localization and origins of juvenile skeletogenic cells in the sea urchin Lytechinuspictus.Developmental biology · 2024Article
- Elongation capacity of polyunsaturated fatty acids in the annelidOpen biology · 2024Article
- Dynamic microvilli sculpt bristles at nanometric scale.Nature communications · 2024Article
- Annelid adult cell type diversity and their pluripotent cellular origins.Nature communications · 2024Article
- Expression of novel androgen receptors in three GnRH neuron subtypes in the cichlid brain.bioRxiv : the preprint server for biology · 2024Article
- Environmental and molecular control of tissue-specific ionocyte differentiation in zebrafish.bioRxiv : the preprint server for biology · 2024Article
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Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Access to newer, fast, and cheap sequencing techniques, particularly on the single-cell level, have made transcriptomic data of tissues or single cells accessible to many researchers. As a consequence, there is an increased need for in situ visualization of gene expression or encoded proteins to validate, localize, or help interpret such sequencing data, as well as put them in context with cellular proliferation. A particular challenge for labeling and imaging transcripts are complex tissues that are often opaque and/or pigmented, preventing easy visual inspection. Here, we introduce a versatile protocol that combines in situ hybridization chain reaction, immunohistochemistry, and proliferative cell labeling using 5-ethynyl-2'-deoxyuridine, and demonstrate its compatibility with tissue clearing. As a proof-of-concept, we show that our protocol allows for the parallel analysis of cell proliferation, gene expression, and protein localization in bristleworm heads and trunks.
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Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.