Evidence map›Paper›PMID 36879194›Full record

ArticleBMC molecular and cell biology2023

Urine-derived mesenchymal stem cells-derived exosomes enhances survival and proliferation of aging retinal ganglion cells.

Qi-Qin Dan, Li Chen, Lan-Lan Shi, Xiu Zhou, Ting-Hua Wang, Hua Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular and cell biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Qi-Qin DanInstitute of Neurological Disease, West China Hospital, Sichuan University, No. 88 Keyuan South Road, Chengdu, 610041, China.
Li ChenInstitute of Neurological Disease, West China Hospital, Sichuan University, No. 88 Keyuan South Road, Chengdu, 610041, China.
Lan-Lan ShiDepartment of Human Anatomy and Tissue Embryology, Kunming Medical University, Kunming, 650500, China.
Xiu ZhouLaboratory Animal Department, Institute of Neuroscience, Kunming Medical University, Kunming, 650500, China.
Ting-Hua WangInstitute of Neurological Disease, West China Hospital, Sichuan University, No. 88 Keyuan South Road, Chengdu, 610041, China. Wangth_email@163.com.
Hua LiuDepartment of Anatomy, Institute of Eyes, Jinzhou Medical University, No.40, Section 3, Songpo Road, Linghe District, JinzhouLiaoning, 121001, China. 1604571378@qq.com.
Kunming Medical University · CNWest China Hospital of Sichuan University · CNJinzhou Medical University · CNSichuan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThis study was designed to investigate to test the effect of exosomes from urine-derived mesenchymal stem cells (USCs) on the survival and viability of aging retinal ganglion cells (RGCs), and explored the preliminary related mechanisms.

methodsPrimary USCs were cultured and identified by immunofluorescence staining. Aging RGCs models were established by D-galactose treatment and identified by β-Galactosidase staining. After treatment with USCs conditioned medium (with USCs removal), flow cytometry was performed to examine the apoptosis and cell cycle of RGCs. Cell viability of RGCs was detected by Cell-counting Kit 8 (CCK8) assay. Moreover, gene sequencing and bioinformatics analysis were applied to analyze the genetic variation after medium treatment in RGCs along with the biological functions of differentially expressed genes (DEGs).

resultsThe number of apoptotic aging RGCs was significantly reduced in USCs medium-treated RGCs. Besides, USCs-derived exosomes exert significant promotion on the cell viability and proliferation of aging RGCs. Further, sequencing data analyzed and identified DEGs expressed in aging RGCs and aging RGCs treated with USCs conditioned medium. The sequencing outcomes demonstrated 117 upregulated genes and 186 downregulated genes in normal RGCs group vs aging RGCs group, 137 upregulated ones and 517 downregulated ones in aging RGCs group vs aging RGCs + USCs medium group. These DEGs involves in numerous positive molecular activities to promote the recovery of RGCs function.

conclusionsCollectively, the therapeutic potentials of USCs-derived exosomes include suppression on cell apoptosis, enhancement on cell viability and proliferation of aging RGCs. The underlying mechanism involves multiple genetic variation and changes of transduction signaling pathways.

Indexed as

ExosomesMesenchymal Stem CellsCell DivisionCulture Media, ConditionedRetinal Ganglion CellsCulture Media, ConditionedAging-related RGCs function lossCell viability and proliferationRetinal ganglion cellsUrine-derived stem cells-derived exosomes

Identifiers

PMID36879194
PMCPMC9990288
OpenAlexW4323321566

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.