Evidence mapPaperPMID 36899839Full record

ArticleCells2023

IL-33 via PKCμ/PRKD1 Mediated α-Catenin Phosphorylation Regulates Endothelial Cell-Barrier Integrity and Ischemia-Induced Vascular Leakage.

Deepti Sharma, Geetika Kaur, Shivantika Bisen, Anamika Sharma, Ahmed S Ibrahim, Nikhlesh K Singh

Open access · goldFull text read
In one paragraph

Article in Cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Allergen-induced activation of epithelial P2YThe Journal of allergy and clinical immunology · 2025
    Article
  7. pH-sensing GPR68 inhibits vascular smooth muscle cell proliferation through Rap1A.American journal of physiology. Heart and circulatory physiology · 2024
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Deepti SharmaIntegrative Biosciences Center, Wayne State University, Detroit, MI 48202, USA.ORCID 0000-0002-1824-6995
Geetika KaurIntegrative Biosciences Center, Wayne State University, Detroit, MI 48202, USA.ORCID 0000-0003-0988-4503
Shivantika BisenIntegrative Biosciences Center, Wayne State University, Detroit, MI 48202, USA.
Anamika SharmaIntegrative Biosciences Center, Wayne State University, Detroit, MI 48202, USA.ORCID 0009-0004-5069-1994
Ahmed S IbrahimDepartment of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48202, USA.ORCID 0000-0001-8480-6252
Nikhlesh K SinghIntegrative Biosciences Center, Wayne State University, Detroit, MI 48202, USA.ORCID 0000-0002-3665-6023
Wayne State University · USMansoura University · EG

Funding

VISION RESEARCH--COREP30EY004068 · WAYNE STATE UNIVERSITY · 1985 to 2025
$2.6M
NEI NIH HHS P30 EY004068NEI NIH HHS R01 EY029709
6 · The paper itself

Abstract

Angiogenesis, neovascularization, and vascular remodeling are highly dynamic processes, where endothelial cell-cell adhesion within the vessel wall controls a range of physiological processes, such as growth, integrity, and barrier function. The cadherin-catenin adhesion complex is a key contributor to inner blood-retinal barrier (iBRB) integrity and dynamic cell movements. However, the pre-eminent role of cadherins and their associated catenins in iBRB structure and function is not fully understood. Using a murine model of oxygen-induced retinopathy (OIR) and human retinal microvascular endothelial cells (HRMVECs), we try to understand the significance of IL-33 on retinal endothelial barrier disruption, leading to abnormal angiogenesis and enhanced vascular permeability. Using electric cell-substrate impedance sensing (ECIS) analysis and FITC-dextran permeability assay, we observed that IL-33 at a 20 ng/mL concentration induced endothelial-barrier disruption in HRMVECs. The adherens junction (AJs) proteins play a prominent role in the selective diffusion of molecules from the blood to the retina and in maintaining retinal homeostasis. Therefore, we looked for the involvement of adherens junction proteins in IL-33-mediated endothelial dysfunction. We observed that IL-33 induces α-catenin phosphorylation at serine/threonine (Ser/Thr) residues in HRMVECs. Furthermore, mass-spectroscopy (MS) analysis revealed that IL-33 induces the phosphorylation of α-catenin at Thr

Indexed as

Endothelial CellsInterleukin-33alpha CateninAnimalsCadherinsHumansIschemiaMiceOxygenp38 Mitogen-Activated Protein KinasesPhosphorylationProtein Kinase Calpha CateninCadherinsIL33 protein, humanIl33 protein, mouseInterleukin-33Oxygenp38 Mitogen-Activated Protein KinasesProtein Kinase Cprotein kinase Dadherens junctioniBRBPKCμ/PRKD1proliferative retinopathyvascular leakageα-catenin

Identifiers

PMID36899839
PMCPMC10001418
OpenAlexW4321597999

What Socratic holds

Textfull text, public
LicenceCC BY
reference markers read17
measurements read29
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.