Evidence map›Paper›PMID 36901715›Full record

ArticleInternational journal of molecular sciences2023

Effects of Long-Term Physical Activity and BCAA Availability on the Subcellular Associations between Intramyocellular Lipids, Perilipins and PGC-1

Vasco Fachada, Mika Silvennoinen, Ulla-Maria Sahinaho, Paavo Rahkila, Riikka Kivelä, Juha J Hulmi, Urho Kujala, Heikki Kainulainen

Open access · goldFull text readTwin Study
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Trial
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Vasco FachadaFaculty of Sport and Health Sciences, NeuroMuscular Research Center, University of Jyväskylä, FI-40014 Jyväskylä, Finland.ORCID 0000-0002-6575-1943
Mika SilvennoinenFaculty of Sport and Health Sciences, NeuroMuscular Research Center, University of Jyväskylä, FI-40014 Jyväskylä, Finland.ORCID 0000-0002-5389-8251
Ulla-Maria SahinahoFaculty of Sport and Health Sciences, NeuroMuscular Research Center, University of Jyväskylä, FI-40014 Jyväskylä, Finland.
Paavo RahkilaFaculty of Sport and Health Sciences, NeuroMuscular Research Center, University of Jyväskylä, FI-40014 Jyväskylä, Finland.
Riikka KiveläFaculty of Sport and Health Sciences, NeuroMuscular Research Center, University of Jyväskylä, FI-40014 Jyväskylä, Finland.ORCID 0000-0002-2686-8890
Juha J HulmiFaculty of Sport and Health Sciences, NeuroMuscular Research Center, University of Jyväskylä, FI-40014 Jyväskylä, Finland.ORCID 0000-0003-3813-2124
Urho KujalaFaculty of Sport and Health Sciences, NeuroMuscular Research Center, University of Jyväskylä, FI-40014 Jyväskylä, Finland.ORCID 0000-0002-9262-1992
Heikki KainulainenFaculty of Sport and Health Sciences, NeuroMuscular Research Center, University of Jyväskylä, FI-40014 Jyväskylä, Finland.ORCID 0000-0001-5102-4350
University of Jyväskylä · FI

Funding

Academy of Finland 114866Academy of Finland 132987Academy of Finland 298875Fundação para a Ciência e Tecnologia SFRH/BD/68308/2010
6 · The paper itself

Abstract

Cellular skeletal muscle lipid metabolism is of paramount importance for metabolic health, specifically through its connection to branched-chain amino acids (BCAA) metabolism and through its modulation by exercise. In this study, we aimed at better understanding intramyocellular lipids (IMCL) and their related key proteins in response to physical activity and BCAA deprivation. By means of confocal microscopy, we examined IMCL and the lipid droplet coating proteins PLIN2 and PLIN5 in human twin pairs discordant for physical activity. Additionally, in order to study IMCLs, PLINs and their association to peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) in cytosolic and nuclear pools, we mimicked exercise-induced contractions in C2C12 myotubes by electrical pulse stimulation (EPS), with or without BCAA deprivation. The life-long physically active twins displayed an increased IMCL signal in type I fibers when compared to their inactive twin pair. Moreover, the inactive twins showed a decreased association between PLIN2 and IMCL. Similarly, in the C2C12 cell line, PLIN2 dissociated from IMCL when myotubes were deprived of BCAA, especially when contracting. In addition, in myotubes, EPS led to an increase in nuclear PLIN5 signal and its associations with IMCL and PGC-1α. This study demonstrates how physical activity and BCAA availability affects IMCL and their associated proteins, providing further and novel evidence for the link between the BCAA, energy and lipid metabolisms.

Indexed as

Amino Acids, Branched-ChainPerilipinsExerciseHumansLipidsMuscle, SkeletalPerilipin-2Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaProteinsAmino Acids, Branched-ChainLipidsPerilipin-2PerilipinsPeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPPARGC1A protein, humanProteinsC2C12electrical pulse stimulationEPSlipid dropletsphysical activityPLIN2PLIN5skeletal musclesubcellular localization

Identifiers

PMID36901715
PMCPMC10002284
OpenAlexW4321499771

What Socratic holds

Textfull text, public
LicenceCC BY
measurements read36
table measurements read24
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.