Evidence map›Paper›PMID 36901985›Full record

ArticleInternational journal of molecular sciences2023

Altered Storage and Function of von Willebrand Factor in Human Cardiac Microvascular Endothelial Cells Isolated from Recipient Transplant Hearts.

Athinoula Meli, Ann McCormack, Ianina Conte, Qu Chen, James Streetley, Marlene L Rose, Ruben Bierings, Matthew J Hannah, Justin E Molloy, Peter B Rosenthal and 1 more

Full text read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Athinoula MeliTransplant Immunology, Heart Science Centre, Harefield Hospital, Hill End Road, Harefield UB9 6JH, UK.
Ann McCormackTransplant Immunology, Heart Science Centre, Harefield Hospital, Hill End Road, Harefield UB9 6JH, UK.
Ianina ConteMolecular and Clinical Sciences Research Institute, St Georges University of London, London SW17 0RE, UK.
Qu ChenStructural Biology Science Technology Platform, The Francis Crick Institute, London NW1 1AT, UK.
James StreetleyStructural Biology of Cells and Viruses Laboratory, The Francis Crick Institute, London NW1 1AT, UK.ORCID 0000-0001-7808-605X
Marlene L RoseTransplant Immunology, Heart Science Centre, Harefield Hospital, Hill End Road, Harefield UB9 6JH, UK.
Ruben BieringsHematology, Erasmus University Medical Center, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands.ORCID 0000-0002-1205-9689
Matthew J HannahHigh Containment Microbiology, UK Health Security Agency, London NW9 5EQ, UK.ORCID 0000-0002-4467-5865
Justin E MolloySingle Molecule Enzymology Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Peter B RosenthalStructural Biology of Cells and Viruses Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Tom CarterMolecular and Clinical Sciences Research Institute, St Georges University of London, London SW17 0RE, UK.ORCID 0000-0002-6248-7278

Funding

British Heart Foundation RG/06/004Landsteiner Stichting voor Bloedtransfusie Research LSBR-1707 and LSBR-2005Medical Research Council MC_PC_13053Wellcome Trust FC001156, FC001143, FC001119
6 · The paper itself

Abstract

The assembly of von Willebrand factor (VWF) into ordered helical tubules within endothelial Weibel-Palade bodies (WPBs) is required for the efficient deployment of the protein at sites of vascular injury. VWF trafficking and storage are sensitive to cellular and environmental stresses that are associated with heart disease and heart failure. Altered storage of VWF manifests as a change in WPB morphology from a rod shape to a rounded shape and is associated with impaired VWF deployment during secretion. In this study, we examined the morphology, ultrastructure, molecular composition and kinetics of exocytosis of WPBs in cardiac microvascular endothelial cells isolated from explanted hearts of patients with a common form of heart failure, dilated cardiomyopathy (DCM; HCMEC

Indexed as

Heart Failurevon Willebrand FactorCells, CulturedEndothelial CellsExocytosisHumansTissue Plasminogen ActivatorTissue Plasminogen Activatorvon Willebrand Factorcardiac microvasculardilated cardiomyopathyendothelial cellsexocytosissecretionvon Willebrand factorWeibel–Palade body

Identifiers

PMID36901985
PMCPMC10003102

What Socratic holds

Textfull text, public
LicenceCC BY
measurements read24
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.