Evidence map›Paper›PMID 36903937›Full record

ArticlePlants (Basel, Switzerland)2023

New Low Morphine Opium Poppy Genotype Obtained by TILLING Approach.

Jiří Červeň, Viktor Vrbovský, Jiří Horáček, Martin Bartas, Lenka Endlová, Petr Pečinka, Vladislav Čurn

Full text read
In one paragraph

Article in Plants (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. White-Seeded Culinary Poppy (Plants (Basel, Switzerland) · 2024
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jiří ČerveňDepartment of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic.
Viktor VrbovskýResearch Institute of Oilseed Crops, Development and Research, Purkyňova 10, 764 01 Opava, Czech Republic.
Jiří HoráčekAgritec Plant Research, Ltd., Zemědělská 2520/16, 787 01 Šumperk, Czech Republic.
Martin BartasDepartment of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic.ORCID 0000-0002-4415-2220
Lenka EndlováResearch Institute of Oilseed Crops, Development and Research, Purkyňova 10, 764 01 Opava, Czech Republic.
Petr PečinkaDepartment of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, 710 00 Ostrava, Czech Republic.ORCID 0000-0003-1270-3646
Vladislav ČurnDepartment of Genetics and Agricultural Biotechnology, Faculty of Agriculture, University of South Bohemia, Studentská 1668, 370 05 České Budějovice, Czech Republic.ORCID 0000-0002-9661-8715

Funding

Ministry of Agriculture of the Czech republic QK1810391University of South Bohemia GAJU080/2022/Z
6 · The paper itself

Abstract

The opium poppy's ability to produce various alkaloids is both useful and problematic. Breeding of new varieties with varying alkaloid content is therefore an important task. In this paper, the breeding technology of new low morphine poppy genotypes, based on a combination of a TILLING approach and single-molecule real-time NGS sequencing, is presented. Verification of the mutants in the TILLING population was obtained using RT-PCR and HPLC methods. Only three of the single-copy genes of the morphine pathway among the eleven genes were used for the identification of mutant genotypes. Point mutations were obtained only in one gene (

Indexed as

chemical mutagenesisexpression profilesmorphine contentnew breeding methodsopium poppyTILLING

Identifiers

PMID36903937
PMCPMC10005565

What Socratic holds

Textfull text, public
LicenceCC BY
reference markers read2
measurements read32
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.