Evidence map›Paper›PMID 36905340›Full record

ArticleBiotechnology journal2023

Microwell-based flow culture increases viability and restores drug response in prostate cancer spheroids.

Marie C Payne, SumYat Ho, Takao Hashimoto, Sara Imboden, Johnny A Diaz, Brandon S Lee, Melissa J Rupert, Nathan Y Cai, Andrew S Goldstein, Neil Y C Lin

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Marie C PayneDepartment of Mechanical & Aerospace Engineering, University of California, Los Angeles, California, USA.ORCID https://orcid.org/0000-0001-8700-2460
SumYat HoDepartment of Biochemistry, University of California, Los Angeles, California, USA.
Takao HashimotoDepartments of Molecular, Cell & Developmental Biology and Urology, University of California, Los Angeles, California, USA.
Sara ImbodenDepartment of Mechanical & Aerospace Engineering, University of California, Los Angeles, California, USA.ORCID https://orcid.org/0000-0002-9185-652X
Johnny A DiazDepartments of Molecular, Cell & Developmental Biology and Urology, University of California, Los Angeles, California, USA.
Brandon S LeeDepartment of Bioengineering, University of California, Los Angeles, California, USA.
Melissa J RupertDepartment of Bioengineering, University of California, Los Angeles, California, USA.
Nathan Y CaiDepartment of Bioengineering, University of California, Los Angeles, California, USA.
Andrew S GoldsteinDepartments of Molecular, Cell & Developmental Biology and Urology, University of California, Los Angeles, California, USA.
Neil Y C LinDepartment of Mechanical & Aerospace Engineering, University of California, Los Angeles, California, USA.
University of California, Los Angeles · USQB3 · US

Funding

UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
UCLA SPORE IN PROSTATE CANCERP50CA092131 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI REITER, ROBERT E · 2002 to 2023
$42.6M
The Origins of Metabolic Reprogramming in Prostate CancerR01CA237191 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GOLDSTEIN, ANDREW S · 2019 to 2023
$2.0M
NCATS NIH HHS UL1 TR001881NCI NIH HHS P50 CA092131NCI NIH HHS R01 CA237191
6 · The paper itself

Abstract

3D cancer spheroids represent a highly promising model for study of cancer progression and therapeutic development. Wide-scale adoption of cancer spheroids, however, remains a challenge due to the lack of control over hypoxic gradients that may cloud the assessment of cell morphology and drug response. Here, we present a Microwell Flow Device (MFD) that generates in-well laminar flow around 3D tissues via repetitive tissue sedimentation. Using a prostate cancer cell line, we demonstrate the spheroids in the MFD exhibit improved cell growth, reduced necrotic core formation, enhanced structural integrity, and downregulated expression of cell stress genes. The flow-cultured spheroids also exhibit an improved sensitivity to chemotherapy with greater transcriptional response. These results demonstrate how fluidic stimuli reveal the cellular phenotype previously masked by severe necrosis. Our platform advances 3D cellular models and enables study into hypoxia modulation, cancer metabolism, and drug screening within pathophysiological conditions.

Indexed as

Prostatic NeoplasmsSpheroids, CellularCell Culture TechniquesDrug Evaluation, PreclinicalHumansMaleflow cultureimagingmillifluidicsprostate cancerspheroidtissue engineering

Identifiers

PMID36905340
PMCPMC12306859
OpenAlexW4323920826

What Socratic holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.