ArticleThe Plant cell2023
Uptake of oomycete RXLR effectors into host cells by clathrin-mediated endocytosis.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Phytophthora targets plant extracellular vesicles to promote infection.Nature microbiology · 2026Article
- Oomycete plant pathogens: biology, pathogenesis and emerging control strategies.Nature reviews. Microbiology · 2026Review
- Clathrin-mediated endocytosis of ERECTA family receptors is essential for proper stomatal development inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- The diverse roles of host membranes in plant-microbe interactions.PLoS pathogens · 2026Article
- Intraspecies sequence-graph analysis of the Phytophthora theobromicola genome reveals a dynamic structure and variable effector repertoires.G3 (Bethesda, Md.) · 2026Article
- Host microenvironment in potato-Phytophthora infestans interaction revealed by single-cell spatiotemporal transcriptome.Nature plants · 2026Article
- Upregulation of deubiquitinase UBP16 induced by rice stripe virus infection stabilizes SHMT1 to suppress ROS accumulation and facilitate virus infection in Nicotiana benthamiana.Stress biology · 2025Article
- Identification of MARVELous Protein Markers for Phytophthora infestans Extracellular Vesicles.Journal of extracellular vesicles · 2025Article
- Structural Insights Into the Role of RxLR Effectors in the Arms Race Between Oomycetes and Plants.Molecular plant pathology · 2025Review
- Phytophthora Disrupts Plant Immunity by Manipulating Nitric Oxide Homeostasis Through GSNOR Inhibition.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Deciphering the Molecular Interplay Between RXLR-EncodedInternational journal of molecular sciences · 2025Review
- Phytophthora Avr3a-Like Effectors Target and Inhibit Cinnamyl Alcohol Dehydrogenase CAD5 to Suppress Plant Immunity.Molecular plant pathology · 2025Article
- Article
- Live cell imaging of plant infection provides new insight into the biology of pathogenesis by the rice blast fungus Magnaporthe oryzae.Journal of microscopy · 2025Review
- Proteolytic processing of both RXLR and EER motifs in oomycete effectors.The New phytologist · 2025Article
- Endoplasmic reticulum homeostasis in plant-pathogen interactions: new scenarios for an old story.Journal of experimental botany · 2025Review
- Immunoisolation of Plant Endosomal Vesicles to Explore Uptake of Pathogen Effector Proteins During Infection of Nicotiana benthamiana.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A pharmacological approach to investigating effector translocation in rice-Plant signaling & behavior · 2024Article
- Transformation-based gene silencing and functional characterization of an ISC effector reveal how a powdery mildew fungus disturbs salicylic acid biosynthesis and immune response in the plant.Molecular plant pathology · 2024Article
- Fungal effectors: past, present, and future.Current opinion in microbiology · 2024Review
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Funding
Abstract
Filamentous (oomycete and fungal) plant pathogens deliver cytoplasmic effector proteins into host cells to facilitate disease. How RXLR effectors from the potato late blight pathogen Phytophthora infestans enter host cells is unknown. One possible route involves clathrin-mediated endocytosis (CME). Transient silencing of NbCHC, encoding clathrin heavy chain, or the endosome marker gene NbAra6 encoding a Rab GTPase in the model host Nicotiana benthamiana, attenuated P. infestans infection and reduced translocation of RXLR effector fusions from transgenic pathogen strains into host cells. By contrast, silencing PP1c isoforms, susceptibility factors not required for endocytosis, reduced infection but did not attenuate RXLR effector uptake. Endosome enrichment by ultracentrifugation and sucrose gradient fractionation revealed co-localization of RXLR effector Pi04314-RFP with clathrin-coated vesicles. Immunopurification of clathrin- and NbAra6-associated vesicles during infection showed that RXLR effectors Pi04314-RFP and AvrBlb1-RFP, but not apoplastic effector PiSCR74-RFP, were co-immunoprecipitated during infection with pathogen strains secreting these effectors. Tandem mass spectrometry analyses of proteins co-immunoprecipitated with NbAra6-GFP during infection revealed enrichment of host proteins associated with endocytic vesicles alongside multiple pathogen RXLR effectors, but not apoplastic effectors, including PiSCR74, which do not enter host cells. Our data show that the uptake of P. infestans RXLR effectors into plant cells occurs via CME.
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