Evidence map›Paper›PMID 36915486›Full record

ArticleHeliyon2023

Multi-omics of NET formation and correlations with CNDP1, PSPB, and L-cystine levels in severe and mild COVID-19 infections.

Lisa M Bramer, Robert D Hontz, Amie J Eisfeld, Amy C Sims, Young-Mo Kim, Kelly G Stratton, Carrie D Nicora, Marina A Gritsenko, Athena A Schepmoes, Osamu Akasaka and 24 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

34 authors at 10 institutions in 2 countries.

Lisa M BramerPacific Northwest National Laboratory, Richland, WA, USA.
Robert D HontzU.S. Naval Medical Research Unit No. TWO (NAMRU-2), Singapore, Singapore.
Amie J EisfeldDepartment of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA.
Amy C SimsPacific Northwest National Laboratory, Richland, WA, USA.
Young-Mo KimPacific Northwest National Laboratory, Richland, WA, USA.
Kelly G StrattonPacific Northwest National Laboratory, Richland, WA, USA.
Carrie D NicoraPacific Northwest National Laboratory, Richland, WA, USA.
Marina A GritsenkoPacific Northwest National Laboratory, Richland, WA, USA.
Athena A SchepmoesPacific Northwest National Laboratory, Richland, WA, USA.
Osamu AkasakaEmergency Medical Center, Fujisawa City Hospital 2-6-1 Fujisawa, Fujisawa, Japan.
Michiko KogaDivision of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Takeya TsutsumiDivision of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Morio NakamuraDepartment of Pulmonary Medicine, Tokyo Saiseikai Central, Tokyo, Japan.
Ichiro NakachiPulmonary Division, Department of Internal Medicine, Utsunomiya Hospital, Utsunomiya, Japan.
Rie BabaPulmonary Division, Department of Internal Medicine, Utsunomiya Hospital, Utsunomiya, Japan.
Hiroki TatenoDepartment of Pulmonary Medicine, Saitama City Hospital, Saitama, Japan.
Shoji SuzukiDepartment of Pulmonary Medicine, Saitama City Hospital, Saitama, Japan.
Hideaki NakajimaDepartment of Hematology and Clinical Immunology, University School of Medicine, Yokohama, Japan.
Hideaki KatoDepartment of Hematology and Clinical Immunology, University School of Medicine, Yokohama, Japan.
Kazunari IshidaKobe Kaisei Hospital, Kobe, Japan.
Makoto IshiiDivision of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo, Japan.
Yoshifumi UwaminoDepartment of Laboratory Medicine, Keio University School of Medicine, Tokyo, Japan.
Keiko MitamuraDivision of Infection Control, Eiju General Hospital, Tokyo, Japan.
Vanessa L PaurusPacific Northwest National Laboratory, Richland, WA, USA.
Ernesto S NakayasuPacific Northwest National Laboratory, Richland, WA, USA.
Isaac K AttahPacific Northwest National Laboratory, Richland, WA, USA.
Andrew G LetiziaU.S. Naval Medical Research Unit No. TWO (NAMRU-2), Singapore, Singapore.
Katrina M WatersPacific Northwest National Laboratory, Richland, WA, USA.
Thomas O MetzPacific Northwest National Laboratory, Richland, WA, USA.
Karen CorsonU.S. Naval Medical Research Unit No. TWO (NAMRU-2), Singapore, Singapore.
Yoshihiro KawaokaDepartment of Pathobiological Sciences, University of Wisconsin-Madison, Madison, WI, USA.
Vincent R GerbasiPacific Northwest National Laboratory, Richland, WA, USA.
Hiroshi YotsuyanagiDivision of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo.
Kiyoko Iwatsuki-HorimotoDivision of Virology, Institute of Medical Science, University of Tokyo, Japan.
Pacific Northwest National Laboratory · USKeio University · JPSaiseikai Utsunomiya hospital · JPSaitama Municipal Hospital · JPUniversity of Wisconsin–Madison · USAdvanced Medical Research Institute · USEiju General Hospital · JPFujisawa City Hospital · JPKobe Kaisei Hospital · JPSaiseikai Central Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The detailed mechanisms of COVID-19 infection pathology remain poorly understood. To improve our understanding of SARS-CoV-2 pathology, we performed a multi-omics and correlative analysis of an immunologically naïve SARS-CoV-2 clinical cohort from blood plasma of uninfected controls, mild, and severe infections. Consistent with previous observations, severe patient populations showed an elevation of pulmonary surfactant levels. Intriguingly, mild patients showed a statistically significant elevation in the carnosine dipeptidase modifying enzyme (CNDP1). Mild and severe patient populations showed a strong elevation in the metabolite L-cystine (oxidized form of the amino acid cysteine) and enzymes with roles in glutathione metabolism. Neutrophil extracellular traps (NETs) were observed in both mild and severe populations, and NET formation was higher in severe vs. mild samples. Our correlative analysis suggests a potential protective role for CNDP1 in suppressing PSPB release from the pulmonary space whereas NET formation correlates with increased PSPB levels and disease severity. In our discussion we put forward a possible model where NET formation drives pulmonary occlusions and CNDP1 promotes antioxidation, pleiotropic immune responses, and vasodilation by accelerating histamine synthesis.

Indexed as

CarnosinaseCarnosineCNDP1COVID-19CystineGlutathioneNETsNeutrophilsROSSARS-CoV-2

Identifiers

PMID36915486
PMCPMC9988701
OpenAlexW4323349230

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.