Evidence map›Paper›PMID 36916875›Full record

ArticleACS applied materials & interfaces2023

Extracellular Matrix-Derived Biophysical Cues Mediate Interstitial Flow-Induced Sprouting Angiogenesis.

Chia-Wen Chang, Hsiu-Chen Shih, Marcos G Cortes-Medina, Peter E Beshay, Alex Avendano, Alex J Seibel, Wei-Hao Liao, Yi-Chung Tung, Jonathan W Song

Open access · greenAbstract read
In one paragraph

Article in ACS applied materials & interfaces, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Chia-Wen ChangDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, United States.ORCID 0000-0002-0810-8556
Hsiu-Chen ShihResearch Center for Applied Science, Academia Sinica, Taipei 115-29, Taiwan.
Marcos G Cortes-MedinaDepartment of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
Peter E BeshayDepartment of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
Alex AvendanoDepartment of Biomedical Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
Alex J SeibelDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, United States.
Wei-Hao LiaoResearch Center for Applied Science, Academia Sinica, Taipei 115-29, Taiwan.
Yi-Chung TungResearch Center for Applied Science, Academia Sinica, Taipei 115-29, Taiwan.ORCID 0000-0002-6170-2992
Jonathan W SongDepartment of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, Ohio 43210, United States.ORCID 0000-0002-6991-5298
The Ohio State University · USResearch Center for Applied Science, Academia Sinica · TW

Funding

Translational Therapeutics Research Program (TT)P30CA016058 · NCI · OHIO STATE UNIVERSITY · PI Amanda Ewart Toland · 1985 to 2026
$132.3M
Biophysical-based approach for controlling blood vessel structure and functionR01HL141941 · NHLBI · OHIO STATE UNIVERSITY · PI CASTRO, CARLOS E., PRAKASH, SHAURYA · 2018 to 2021
$2.0M
NCI NIH HHS P30 CA016058NHLBI NIH HHS R01 HL141941
6 · The paper itself

Abstract

Sprouting angiogenesis is orchestrated by an intricate balance of biochemical and mechanical cues in the local tissue microenvironment. Interstitial flow has been established as a potent regulator of angiogenesis. Similarly, extracellular matrix (ECM) physical properties, such as stiffness and microarchitecture, have also emerged as important mediators of angiogenesis. However, the interplay between interstitial flow and ECM physical properties in the initiation and control of angiogenesis is poorly understood. Using a three-dimensional (3D) microfluidic tissue analogue of angiogenic sprouting with defined interstitial flow superimposed over ECM with well-characterized physical properties, we found that the addition of hyaluronan (HA) to collagen-based matrices significantly enhances sprouting induced by interstitial flow compared to responses in collagen-only hydrogels. We confirmed that both the stiffness and matrix pore size of collagen-only hydrogels were increased by the addition of HA. Interestingly, interstitial flow-potentiated sprouting responses in collagen/HA matrices were not affected when functionally blocking the HA receptor CD44. In contrast, enzymatic depletion of HA in collagen/HA matrices with hyaluronidase (HAdase) resulted in decreased stiffness, pore size, and interstitial flow-mediated sprouting to the levels observed in collagen-only matrices. Taken together, these results suggest that HA enhances interstitial flow-mediated angiogenic sprouting through its alterations to collagen ECM stiffness and pore size.

Indexed as

CuesExtracellular MatrixCardiovascular Physiological PhenomenaCollagenHydrogelsCollagenHydrogelshyaluronidasemicrofluidicsmicrovessel analoguepore sizestiffnessvascular function

Identifiers

PMID36916875
PMCPMC11078157
OpenAlexW4324129980

What Socratic holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.