Evidence map›Paper›PMID 36920219›Full record

ArticlemSphere2023

An APOBEC3 Mutational Signature in the Genomes of Human-Infecting Orthopoxviruses.

Diego Forni, Rachele Cagliani, Uberto Pozzoli, Manuela Sironi

Open access · goldAbstract read
In one paragraph

Article in mSphere, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 40 citations in OpenAlex.

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  19. Multiple introductions of monkeypox virus to Ireland during the international mpox outbreak, May 2022 to October 2023.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Diego ForniBioinformatics, IRCCS E. Medea Scientific Institute, Bosisio Parini, Lecco, Italy.ORCID 0000-0001-9291-5352
Rachele CaglianiBioinformatics, IRCCS E. Medea Scientific Institute, Bosisio Parini, Lecco, Italy.ORCID 0000-0003-2670-3532
Uberto PozzoliBioinformatics, IRCCS E. Medea Scientific Institute, Bosisio Parini, Lecco, Italy.
Manuela SironiBioinformatics, IRCCS E. Medea Scientific Institute, Bosisio Parini, Lecco, Italy.
IRCCS Eugenio Medea · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ongoing worldwide monkeypox outbreak is caused by viral lineages (globally referred to as hMPXV1) that are related to but distinct from clade IIb MPXV viruses transmitted within Nigeria. Analysis of the genetic differences has indicated that APOBEC-mediated editing might be responsible for the unexpectedly high number of mutations observed in hMPXV1 genomes. Here, using 1,624 publicly available hMPXV1 sequences, we analyzed the mutations that accrued between 2017 and the emergence of the current predominant variant (B.1), as well as those that that have been accumulating during the 2022 outbreak. We confirmed an overwhelming prevalence of C-to-T and G-to-A mutations, with a sequence context (5'-TC-3') consistent with the preferences of several human APOBEC3 enzymes. We also found that mutations preferentially occur in highly expressed viral genes, although no transcriptional asymmetry was observed. A comparison of the mutation spectrum and context was also performed against the human-specific variola virus (VARV) and the zoonotic cowpox virus (CPXV), as well as fowlpox virus (FWPV). The results indicated that in VARV genomes, C-to-T and G-to-A changes were more common than the opposite substitutions, although the effect was less marked than for hMPXV1. Conversely, no preference toward C-to-T and G-to-A changes was observed in CPXV and FWPV. Consistently, the sequence context of C-to-T changes confirmed a preference for a T in the -1 position for VARV, but not for CPXV or FWPV. Overall, our results strongly support the view that, irrespective of the transmission route, orthopoxviruses infecting humans are edited by the host APOBEC3 enzymes.

Indexed as

Mpox, MonkeypoxOrthopoxvirusSmallpoxVariola virusAnimalsAPOBEC DeaminasesCowpox virusHumansMutationAPOBEC3 proteins, humanAPOBEC DeaminasesAPOBECmonkeypoxorthopoxvirussmallpoxvariola virus

Identifiers

PMID36920219
PMCPMC10117092
OpenAlexW4324304910

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.