Evidence mapPaperPMID 36928429Full record

ArticleNature biotechnology2023

Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data.

George I Austin, Heekuk Park, Yoli Meydan, Dwayne Seeram, Tanya Sezin, Yue Clare Lou, Brian A Firek, Michael J Morowitz, Jillian F Banfield, Angela M Christiano and 4 more

Open access · greenAbstract read
In one paragraph

Article in Nature biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
10.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 69 citations in OpenAlex.

  1. Trial
  2. Trial
  3. Trial
  4. Review
  5. Review
  6. Review
  7. Identify contaminants with decontam on the QIIME 2 Framework.Microbiology resource announcements · 2026
    Article
  8. Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 7 institutions in 2 countries.

George I AustinDepartment of Computer Science, Columbia University, New York, NY, USA.
Heekuk ParkDivision of Infectious Diseases, Columbia University Irving Medical Center, New York, NY, USA.ORCID 0000-0001-5815-9717
Yoli MeydanProgram for Mathematical Genomics, Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.
Dwayne SeeramDivision of Infectious Diseases, Columbia University Irving Medical Center, New York, NY, USA.
Tanya SezinDepartment of Dermatology, Columbia University Irving Medical Center, New York, NY, USA.
Yue Clare LouDepartment of Plant and Microbial Biology, University of California, Berkeley, CA, USA.ORCID 0000-0002-0340-2166
Brian A FirekDepartment of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Michael J MorowitzDepartment of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Jillian F BanfieldDepartment of Earth and Planetary Science, University of California, Berkeley, CA, USA.ORCID 0000-0001-8203-8771
Angela M ChristianoDepartment of Dermatology, Columbia University Irving Medical Center, New York, NY, USA.ORCID 0000-0002-6071-9784
Itsik Pe'erDepartment of Computer Science, Columbia University, New York, NY, USA.
Anne-Catrin UhlemannDivision of Infectious Diseases, Columbia University Irving Medical Center, New York, NY, USA.
Liat Shenhav *Center for Studies in Physics and Biology, Rockefeller University, New York, NY, USA. lshenhav@rockefeller.edu.ORCID 0000-0003-1708-6050
Tal Korem *Program for Mathematical Genomics, Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA. tal.korem@columbia.edu.ORCID 0000-0002-0609-0858
Columbia University Irving Medical Center · USColumbia University · USUniversity of Pittsburgh · USCanadian Institute for Advanced Research · CAPlanetary Science Institute · USRockefeller University · USUniversity of California, Berkeley · US

Funding

A large scale investigation of the vaginal metagenome and metabolome and their role in spontaneous preterm birthR01HD106017 · NICHD · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI KOREM, TAL · 2021 to 2025
$3.6M
The role of the microbiome in HPV-associated cervical cancer in women with HIVR01CA245894 · NCI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI UHLEMANN, ANNE-CATRIN · 2020 to 2024
$2.3M
NCI NIH HHS R01 CA245894NICHD NIH HHS R01 HD106017
6 · The paper itself

Abstract

Sequencing-based approaches for the analysis of microbial communities are susceptible to contamination, which could mask biological signals or generate artifactual ones. Methods for in silico decontamination using controls are routinely used, but do not make optimal use of information shared across samples and cannot handle taxa that only partially originate in contamination or leakage of biological material into controls. Here we present Source tracking for Contamination Removal in microBiomes (SCRuB), a probabilistic in silico decontamination method that incorporates shared information across multiple samples and controls to precisely identify and remove contamination. We validate the accuracy of SCRuB in multiple data-driven simulations and experiments, including induced contamination, and demonstrate that it outperforms state-of-the-art methods by an average of 15-20 times. We showcase the robustness of SCRuB across multiple ecosystems, data types and sequencing depths. Demonstrating its applicability to microbiome research, SCRuB facilitates improved predictions of host phenotypes, most notably the prediction of treatment response in melanoma patients using decontaminated tumor microbiome data.

Indexed as

MicrobiotaNeoplasmsHumansPhenotype

Identifiers

PMID36928429
PMCPMC10504420
OpenAlexW4327569177

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.