Evidence map›Paper›PMID 36934171›Full record

ArticleScientific reports2023

qPCR detection of viable Bacillus cereus group cells in cosmetic products.

Nadine Yossa, Sonny Huang, Travis Canida, Rachel Binet, Dumitru Macarisin, Rebecca Bell, Sandra Tallent, Eric Brown, Thomas Hammack

Open access · goldFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Nadine YossaFDA, Office of Regulatory Science, College Park, MD, USA. IreneNadine.Tchagou@fda.hhs.gov.
Sonny HuangOak Ridge Institute for Science and Education, Oak Ridge, TN, 37831, USA.
Travis CanidaFDA, Office of Analytics and Outreach, College Park, MD, 20740, USA.
Rachel BinetFDA, Office of Regulatory Science, College Park, MD, USA.
Dumitru MacarisinFDA, Office of Regulatory Science, College Park, MD, USA.
Rebecca BellFDA, Office of Regulatory Science, College Park, MD, USA.
Sandra TallentFDA, Office of Regulatory Science, College Park, MD, USA.
Eric BrownFDA, Office of Regulatory Science, College Park, MD, USA.
Thomas HammackFDA, Office of Regulatory Science, College Park, MD, USA. Thomas.Hammack@fda.hhs.gov.
United States Food and Drug Administration · USOak Ridge Institute for Science and EducationOffice of Science · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reference methods for microbiological safety assessments of cosmetics rely on culture methods that reveal colonies of live microorganisms on growth media. Rapid molecular technologies, such as qPCR, detects the presence of target DNA in samples from dead and viable cells. DNA intercalating dyes, such as propidium monoazide (PMAxx), are capable of restricting PCR amplification to viable microbial cells. Here we developed singleplex and multiplex real time (qPCR) assays for the detection of Bacillus cereus (B. cereus) using 16S rRNA and phosphatidylcholine-specific phospholipase C (PLC) gene specific sequences coupled with PMAxx. The limit of detection was determined to be ~ 1 log CFU/ml for 16S rRNA and 3 log CFU/ml for PLC detection in pure culture using an eye shadow isolate, B. cereus 3A. We assessed the inclusivity and exclusivity of our qPCR assays using 212 strains, including 143 members of B. cereus, 38 non- B. cereus. and 31 non-Bacillus species; inclusivity was 100% for the 16S rRNA and 97.9% for the PLC targets; the exclusivity was 100% for 16S rRNA and 98.6% for PLC targets. These qPCR assays were then used to assess samples of commercial cosmetics: one set of liquid face toners (N = 3), artificially contaminated with B. cereus 3A, and one set of powdered cosmetics (N = 8), previously determined to be contaminated with B. cereus. For some samples, test portions were analyzed by qPCR in parallel, with and without PMAxx treatment. All test portions were simultaneously streaked on BACARA plates to confirm viable cells of B. cereus, according to the culture method. We found no difference in sensitivity between the singleplex and the multiplex qPCR assays (P > 0.05). Inoculated samples that did not recover B. cereus on plates still showed amplification of the DNA targets. However, that amplification was significantly delayed in PMAxx -treated samples (P < 0.0001) with C

Indexed as

BacillusCosmeticsBacillus cereusColoring AgentsFood MicrobiologyReal-Time Polymerase Chain ReactionRNA, Ribosomal, 16SColoring AgentsCosmeticsRNA, Ribosomal, 16S

Identifiers

PMID36934171
PMCPMC10024758
OpenAlexW4327857161

What Socratic holds

Textfull text, public
LicenceCC BY
measurements read41
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.