Evidence map›Paper›PMID 36945537›Full record

ArticlebioRxiv : the preprint server for biology2023

Generation of Schwann cell derived melanocytes from hPSCs identifies pro-metastatic factors in melanoma.

Ryan M Samuel, Albertas Navickas, Ashley Maynard, Eliza A Gaylord, Kristle Garcia, Samyukta Bhat, Homa Majd, Mikayla N Richter, Nicholas Elder, Daniel Le and 8 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 5 institutions in 3 countries.

Ryan M SamuelDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Albertas NavickasDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
Ashley MaynardChan Zuckerberg Biohub, San Francisco, CA, USA.
Eliza A GaylordEli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA.
Kristle GarciaDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
Samyukta BhatEli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA.
Homa MajdDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Mikayla N RichterDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Nicholas ElderDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
Daniel LeChan Zuckerberg Biohub, San Francisco, CA, USA.
Phi NguyenDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
Bradley ShibataBiological Electron Microscopy Facility, University of California, Davis, CA 95616, USA.
Marta Losa LlabataProgram in Craniofacial Biology, University of California, San Francisco, San Francisco, CA 94110, USA.
Licia SelleriProgram in Craniofacial Biology, University of California, San Francisco, San Francisco, CA 94110, USA.
Diana J LairdEli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA, 94143, USA.
Spyros DarmanisChan Zuckerberg Biohub, San Francisco, CA, USA.
Hani GoodarziDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
Faranak FattahiDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
University of California, San Francisco · USChan Zuckerberg Initiative (United States) · USCentre National de la Recherche Scientifique · FRETH Zurich · CHUniversity of California, Davis · US

Funding

Translational InformaticsP30CA082103 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Alan Ashworth · 1999 to 2026
$209.7M
DIABETES, ENDOCRINOLOGY &METABOLISM TRAINING PROGRAMT32DK007418 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI EDWARD C HSIAO · 1986 to 2026
$14.3M
UCSF Environmental Research and Translation for Health Center (EaRTH Center)P30ES030284 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Rashmi Joglekar · 2020 to 2026
$11.2M
PREDOCTORAL TRAINING IN DEVELOPMENTAL BIOLOGYT32HD007470 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Todd Nystul · 1994 to 2026
$7.7M
Modeling Neurodegenerative Disease Manifestations in Human Enteric NeuronsDP2NS116769 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FATTAHI, FARANAK · 2019 to 2019
$2.4M
Human pluripotent stem cells for the study of gastrointestinal dysmotilityR01DK121169 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FATTAHI, FARANAK · 2020 to 2024
$1.8M
The RNA structural code underlying pathological regulation of RNA splicing in metastasisR01CA244634 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GOODARZI, HANI · 2021 to 2025
$1.8M
Fmr1 Function and Repeat Expansion in the Developing GermlineR01GM122902 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LAIRD, DIANA J · 2018 to 2021
$1.8M
The intersection of stress and environmental chemicals in germ cell reprogrammingR01ES028212 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LAIRD, DIANA J · 2017 to 2021
$1.7M
The Role and Branching Dynamics of Sympathetic Nerves in Ovarian FolliculogenesisF31HD108875 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GAYLORD, ELIZA ANN · 2023 to 2025
$111k
NCI NIH HHS P30 CA082103NCI NIH HHS R01 CA244634NICHD NIH HHS F31 HD108875NICHD NIH HHS T32 HD007470NIDDK NIH HHS R01 DK121169NIDDK NIH HHS T32 DK007418NIEHS NIH HHS P30 ES030284NIEHS NIH HHS R01 ES028212NIGMS NIH HHS R01 GM122902NINDS NIH HHS DP2 NS116769
6 · The paper itself

Abstract

The neural crest (NC) is highly multipotent and generates diverse lineages in the developing embryo. However, spatiotemporally distinct NC populations display differences in fate potential, such as increased gliogenic and parasympathetic potential from later migrating, nerve-associated Schwann cell precursors (SCPs). Interestingly, while melanogenic potential is shared by both early migrating NC and SCPs, differences in melanocyte identity resulting from differentiation through these temporally distinct progenitors have not been determined. Here, we leverage a human pluripotent stem cell (hPSC) model of NC temporal patterning to comprehensively characterize human NC heterogeneity, fate bias, and lineage development. We captured the transition of NC differentiation between temporally and transcriptionally distinct melanogenic progenitors and identified modules of candidate transcription factor and signaling activity associated with this transition. For the first time, we established a protocol for the directed differentiation of melanocytes from hPSCs through a SCP intermediate, termed trajectory 2 (T2) melanocytes. Leveraging an existing protocol for differentiating early NC-derived melanocytes, termed trajectory 1 (T1), we performed the first comprehensive comparison of transcriptional and functional differences between these distinct melanocyte populations, revealing differences in pigmentation and unique expression of transcription factors, ligands, receptors and surface markers. We found a significant link between the T2 melanocyte transcriptional signature and decreased survival in melanoma patients in the cancer genome atlas (TCGA). We performed an

Identifiers

PMID36945537
PMCPMC10028814
OpenAlexW4323350077

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.