Evidence mapPaperPMID 36949528Full record

ArticleStem cell research & therapy2023

Diabetic kidney disease induces transcriptome alterations associated with angiogenesis activity in human mesenchymal stromal cells.

Xiaohui Bian, Sabena M Conley, Alfonso Eirin, Eric A Zimmerman Zuckerman, Anastasia L Smith, Cody C Gowan, Zachary K Snow, Tambi Jarmi, Houssam Farres, Young M Erben and 7 more

Open access · goldFull text read
In one paragraph

Article in Stem cell research & therapy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 7 institutions in 3 countries.

Xiaohui Bian *Division of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 4500 San Pablo Road, Jacksonville, FL, 32224, USA.
Sabena M Conley *Division of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 4500 San Pablo Road, Jacksonville, FL, 32224, USA.
Alfonso EirinDivision of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, Rochester, MN, USA.
Eric A Zimmerman ZuckermanDepartment of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
Anastasia L SmithDivision of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 4500 San Pablo Road, Jacksonville, FL, 32224, USA.
Cody C GowanDivision of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 4500 San Pablo Road, Jacksonville, FL, 32224, USA.
Zachary K SnowDivision of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 4500 San Pablo Road, Jacksonville, FL, 32224, USA.
Tambi JarmiDivision of Transplant Nephrology, Department of Transplant Surgery, Mayo Clinic, Jacksonville, FL, USA.
Houssam FarresDivision of Vascular Surgery, Department of Surgery, Mayo Clinic, Jacksonville, FL, USA.
Young M ErbenDivision of Vascular Surgery, Department of Surgery, Mayo Clinic, Jacksonville, FL, USA.
Albert G HakaimDivision of Vascular Surgery, Department of Surgery, Mayo Clinic, Jacksonville, FL, USA.
Matthew A DietzDepartment of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USA.
Abba C ZubairDepartment of Laboratory Medicine and Pathology, Mayo Clinic, Jacksonville, FL, USA.
Saranya P WylesDepartment of Dermatology, Mayo Clinic, Rochester, MN, USA.
Joy V WolframDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Jacksonville, FL, USA.
Lilach O LermanDivision of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, Rochester, MN, USA.
LaTonya J HicksonDivision of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 4500 San Pablo Road, Jacksonville, FL, 32224, USA. Hickson.LaTonya@mayo.edu.ORCID 0000-0002-7485-336X
Mayo Clinic in Florida · USJackson Memorial Hospital · USMayo Clinic in Arizona · USMayo Clinic · USJacksonville College · USThe University of Queensland · AUWinnMed · US

Funding

NIDDK NIH HHS K23 DK109134NIDDK NIH HHS R03 DK123492
6 · The paper itself

Abstract

backgroundTherapeutic interventions that optimize angiogenic activities may reduce rates of end-stage kidney disease, critical limb ischemia, and lower extremity amputations in individuals with diabetic kidney disease (DKD). Infusion of autologous mesenchymal stromal cells (MSC) is a promising novel therapy to rejuvenate vascular integrity. However, DKD-related factors, including hyperglycemia and uremia, might alter MSC angiogenic repair capacity in an autologous treatment approach.

methodsTo explore the angiogenic activity of MSC in DKD, the transcriptome of adipose tissue-derived MSC obtained from DKD subjects was compared to age-matched controls without diabetes or kidney impairment. Next-generation RNA sequencing (RNA-seq) was performed on MSC (DKD n = 29; Controls n = 9) to identify differentially expressed (DE; adjusted p < 0.05, |log

resultsRNA-seq analyses revealed 133 DE mRNAs (77 upregulated and 56 down-regulated) and 208 DE miRNAs (119 up- and 89 down-regulated) in DKD-MSC versus Control-MSC. Interestingly, miRNA let-7a-5p, which regulates angiogenesis and participates in DKD pathogenesis, interacted with 5 angiogenesis-associated mRNAs (transgelin/TAGLN, thrombospondin 1/THBS1, lysyl oxidase-like 4/LOXL4, collagen 4A1/COL4A1 and collagen 8A1/COL8A1). DKD-MSCcm incubation with injured endothelial cells improved tube formation capacity, enhanced migration, reduced adhesion molecules E-selectin, vascular cell adhesion molecule 1 and intercellular adhesion molecule 1 mRNA expression in endothelial cells. Moreover, angiogenic repair effects did not differ between treatment groups (DKD-MSCcm vs. Control-MSCcm).

conclusionsMSC from individuals with DKD show angiogenic transcriptome alterations compared to age-matched controls. However, angiogenic repair potential may be preserved, supporting autologous MSC interventions to treat conditions requiring enhanced angiogenic activities such as DKD, diabetic foot ulcers, and critical limb ischemia.

Indexed as

Diabetes MellitusDiabetic NephropathiesMesenchymal Stem CellsMicroRNAsChronic Limb-Threatening IschemiaHumansHuman Umbilical Vein Endothelial CellsNeovascularization, PhysiologicProtein-Lysine 6-OxidaseRNA, MessengerTranscriptomeLOXL4 protein, humanMicroRNAsProtein-Lysine 6-OxidaseRNA, MessengerChronic kidney diseaseDiabetes mellitusDiabetic nephropathyIschemic limb diseaseMesenchymal stromal cellsRegenerative medicine

Identifiers

PMID36949528
PMCPMC10035152
OpenAlexW4353092947

What Socratic holds

Textfull text, public
LicenceCC BY
measurements read47
table measurements read7
identifiers read17
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.