Evidence map›Paper›PMID 36972674›Full record

ArticlePoultry science2023

In silico prediction and expression analysis of vaccine candidate genes of Campylobacter jejuni.

Sabin Poudel, Linan Jia, Mark A Arick, Chuan-Yu Hsu, Adam Thrash, Anuraj T Sukumaran, Pratima Adhikari, Aaron S Kiess, Li Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Evaluation of Two Recombinant Protein-Based Vaccine Regimens againstAnimals : an open access journal from MDPI · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Sabin PoudelDepartment of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USA.
Linan JiaDepartment of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USA.
Mark A ArickInstitute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA.
Chuan-Yu HsuInstitute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA.
Adam ThrashInstitute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS 39762, USA.
Anuraj T SukumaranDepartment of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USA.
Pratima AdhikariDepartment of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USA.
Aaron S KiessPrestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA.
Li ZhangDepartment of Poultry Science, Mississippi State University, Mississippi State, MS 39762, USA. Electronic address: l.zhang@msstate.edu.
Mississippi State University · USNorth Carolina State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Campylobacter jejuni (C. jejuni) is the most common food-borne pathogen that causes human gastroenteritis in the United States. Consumption of contaminated poultry products is considered as the major source of human Campylobacter infection. An effective vaccine would be a promising alternative to antibiotic supplements to curb C. jejuni colonization in poultry gastrointestinal (GI) tract. However, the genetic diversity among the C. jejuni isolates makes vaccine production more challenging. Despite many attempts, an effective Campylobacter vaccine is not yet available. This study aimed to identify suitable candidates to develop a subunit vaccine against C. jejuni, which could reduce colonization in the GI tract of the poultry. In the current study, 4 C. jejuni strains were isolated from retail chicken meat and poultry litter samples and their genomes were sequenced utilizing next-generation sequencing technology. The genomic sequences of C. jejuni strains were screened to identify potential antigens utilizing the reverse vaccinology approach. In silico genome analysis predicted 3 conserved potential vaccine candidates (phospholipase A [PldA], TonB dependent vitamin B12 transporter [BtuB], and cytolethal distending toxin subunit B [CdtB]) suitable for the development of a vaccine. Furthermore, the expression of predicted genes during host-pathogen interaction was analyzed by an infection study using an avian macrophage-like immortalized cell line (HD11). The HD11 was infected with C. jejuni strains, and the RT-qPCR assay was performed to determine the expression of the predicted genes. The expression difference was analyzed using ΔΔCt methods. The results indicate that all 3 predicted genes, PldA, BtuB, and CdtB, were upregulated in 4 tested C. jejuni strains irrespective of their sources of isolation. In conclusion, in silico prediction and gene expression analysis during host-pathogen interactions identified 3 potential vaccine candidates for C. jejuni.

Indexed as

CampylobacterCampylobacter InfectionsCampylobacter jejuniVaccinesAnimalsChickensGenes, BacterialHumansPoultryVaccineshost-pathogen interactionpoultryreverse vaccinology, Campylobacter jejuniRT-qPCR

Identifiers

PMID36972674
PMCPMC10066559
OpenAlexW4320914996

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.