ArticleNature methods2023
Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins.
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Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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85citing papers in PubMed
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85 citing papers in PubMed.
- Benchmarking dynamic analysis methods in diffusion-based single-molecule FRET.Cell reports methods · 2026Article
- BRET-Based Approaches for Ion Channels: Emerging Applications and Future Directions.Archiv der Pharmazie · 2026Review
- ATP-driven conformational dynamics reveal hidden intermediates in a heterodimeric ABC transporter.eLife · 2026Article
- Label-Free Single Protein Dynamics Revealed by Metasurface-Enhanced Raman Spectroscopy.ACS nano · 2026Article
- A Universal Protein Ladder for Standardization of Diverse FRET Assays.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Challenging the dry core paradigm: hydrated reactivity emerges within micellar cores.Chemical science · 2026Article
- A Standardized Prism-Based TIRF Platform for Quantitative Single-Molecule Fluorescence Studies of Biomolecular Dynamics.Biosensors · 2026Article
- Dissecting mechanisms of ligand binding and conformational changes in the glutamine-binding protein.eLife · 2026Article
- Advancements in single-molecule fluorescence spectroscopy for probing conformations, dynamics, and interactions in disordered protein regions.Current opinion in structural biology · 2026Review
- A combined PIE-FRET and FCS assay to monitor RNA dynamics and cleavage by SARS-CoV-2 Nsp15.Scientific reports · 2026Article
- Cellular context and ligand class shape CXCR4-CCR5 heteromerization in live cells.Research square · 2026Article
- Strategies for constructing context-specific protein-protein interaction networks.Briefings in bioinformatics · 2026Review
- Single-Molecule Forster Resonance Energy Transfer With a Minimalistic 3D-Printed Setup and Dyes in the Blue-Green Spectral Region.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- DRUMBEAT temporally resolved interpretable machine learning model for characterizing state transitions in protein dynamics.Communications biology · 2026Article
- Resolving fluorescently labeled species using highly multiplexed spectral FLIM.Scientific reports · 2026Article
- Fluorescence bioimaging of drug nanocarriers based on Förster resonance energy transfer, aggregation-induced emission and aggregation-caused quenching.Acta pharmaceutica Sinica. B · 2026Review
- Unraveling allosteric signaling of G protein-coupled receptors (GPCRs) by single-molecule fluorescence.Biophysical reviews · 2026Review
- Single-Molecule Conductance of Non-Redox Proteins: Mechanisms, Measurements, and Applications.Biomolecules · 2026Review
- Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo.iScience · 2026Article
- Simulating Freely Diffusing Single-Molecule FRET Data with Consideration of Protein Conformational Dynamics.The journal of physical chemistry. B · 2026Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
47 authors.
Ganesh Agam *Department of Chemistry, Ludwig-Maximilians University München, München, Germany.ORCID http://orcid.org/0000-0003-1623-4920
Christian Gebhardt *Physical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians University München, Planegg-Martinsried, Germany.ORCID http://orcid.org/0000-0002-5834-6579
Milana Popara *Molecular Physical Chemistry, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.ORCID http://orcid.org/0000-0003-2626-6096
Rebecca MächtelPhysical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians University München, Planegg-Martinsried, Germany.
Julian FolzMolecular Physical Chemistry, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.ORCID http://orcid.org/0000-0001-8232-8787
Benjamin AmbroseDepartment of Chemistry, University of Sheffield, Sheffield, UK.ORCID http://orcid.org/0000-0002-5046-2532
Neharika ChamachiB CUBE - Center for Molecular Bioengineering, Technische Universität Dresden, Dresden, Germany.ORCID http://orcid.org/0000-0002-4657-9092
Sang Yoon ChungDepartment of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.
Timothy D CraggsDepartment of Chemistry, University of Sheffield, Sheffield, UK.ORCID http://orcid.org/0000-0002-7121-0609
Marijn de BoerMolecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, AG Groningen, the Netherlands.
Dina GrohmannDepartment of Biochemistry, Genetics and Microbiology, Institute of Microbiology, Single-Molecule Biochemistry Laboratory, University of Regensburg, Regensburg, Germany.ORCID http://orcid.org/0000-0002-0570-2517
Taekjip HaDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine and Howard Hughes Medical Institute, Baltimore, MD, USA.ORCID http://orcid.org/0000-0003-2195-6258
Andreas HartmannB CUBE - Center for Molecular Bioengineering, Technische Universität Dresden, Dresden, Germany.ORCID http://orcid.org/0000-0002-2213-2763
Jelle HendrixDynamic Bioimaging Laboratory, Advanced Optical Microscopy Center and Biomedical Research Institute, Hasselt University, Agoralaan C (BIOMED), Hasselt, Belgium.ORCID http://orcid.org/0000-0001-5731-1297
Verena HirschfeldInstitute of Physics, University of Lübeck, Lübeck, Germany.
Christian G HübnerInstitute of Physics, University of Lübeck, Lübeck, Germany.
Thorsten HugelInstitute of Physical Chemistry, University of Freiburg, Freiburg, Germany.ORCID http://orcid.org/0000-0003-3292-4569
Dominik KammererDepartment of Physics, Clarendon Laboratory, University of Oxford, Oxford, UK.
Hyun-Seo KangBayerisches NMR Zentrum, Department of Bioscience, School of Natural Sciences, Technical University of München, Garching, Germany.ORCID http://orcid.org/0000-0003-4029-5096
Achillefs N KapanidisDepartment of Physics, Clarendon Laboratory, University of Oxford, Oxford, UK.
Georg KrainerB CUBE - Center for Molecular Bioengineering, Technische Universität Dresden, Dresden, Germany.ORCID http://orcid.org/0000-0002-9626-7636
Kevin KrammDepartment of Biochemistry, Genetics and Microbiology, Institute of Microbiology, Single-Molecule Biochemistry Laboratory, University of Regensburg, Regensburg, Germany.
Edward A LemkeBiocenter, Johannes Gutenberg University Mainz, Mainz, Germany.ORCID http://orcid.org/0000-0002-0634-0503
Eitan LernerDepartment of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, and The Center for Nanoscience and Nanotechnology, Faculty of Mathematics and Science, The Edmond J. Safra Campus, The Hebrew University of Jerusalem, Jerusalem, Israel.ORCID http://orcid.org/0000-0002-3791-5277
Emmanuel MargeatCentre de Biologie Structurale (CBS), University of Montpellier, CNRS, INSERM, Montpellier, France.ORCID http://orcid.org/0000-0001-6063-6420
Kirsten MartensBiological and Soft Matter Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, Leiden, the Netherlands.
Jens MichaelisInstitute for Biophysics, Ulm University, Ulm, Germany.ORCID http://orcid.org/0000-0002-2739-4172
Jaba MitraDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine and Howard Hughes Medical Institute, Baltimore, MD, USA.
Gabriel G Moya MuñozPhysical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians University München, Planegg-Martinsried, Germany.ORCID http://orcid.org/0000-0003-2797-2005
Robert B QuastCentre de Biologie Structurale (CBS), University of Montpellier, CNRS, INSERM, Montpellier, France.ORCID http://orcid.org/0000-0001-8166-6952
Nicole C RobbDepartment of Physics, Clarendon Laboratory, University of Oxford, Oxford, UK.
Michael SattlerBayerisches NMR Zentrum, Department of Bioscience, School of Natural Sciences, Technical University of München, Garching, Germany.
Michael SchlierfB CUBE - Center for Molecular Bioengineering, Technische Universität Dresden, Dresden, Germany.ORCID http://orcid.org/0000-0002-6209-2364
Jonathan SchneiderPhysical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians University München, Planegg-Martinsried, Germany.
Tim SchröderDepartment of Chemistry, Ludwig-Maximilians University München, München, Germany.ORCID http://orcid.org/0000-0002-2474-5395
Anna SeferInstitute for Biophysics, Ulm University, Ulm, Germany.
Piau Siong TanBiocenter, Johannes Gutenberg University Mainz, Mainz, Germany.
Johann ThurnInstitute of Physical Chemistry, University of Freiburg, Freiburg, Germany.ORCID http://orcid.org/0000-0001-6370-7052
Philip TinnefeldDepartment of Chemistry, Ludwig-Maximilians University München, München, Germany.
John van NoortBiological and Soft Matter Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, Leiden, the Netherlands.ORCID http://orcid.org/0000-0002-9094-5598
Shimon WeissDepartment of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-0720-5426
Nicolas WendlerPhysical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians University München, Planegg-Martinsried, Germany.ORCID http://orcid.org/0000-0002-5436-3929
Niels ZijlstraPhysical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians University München, Planegg-Martinsried, Germany.
Anders BarthMolecular Physical Chemistry, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany. a.barth@tudelft.nl.ORCID http://orcid.org/0000-0003-3671-3072
Claus A M SeidelMolecular Physical Chemistry, Heinrich-Heine University Düsseldorf, Düsseldorf, Germany. cseidel@hhu.de.ORCID http://orcid.org/0000-0002-5171-149X
Don C LambDepartment of Chemistry, Ludwig-Maximilians University München, München, Germany. d.lamb@lmu.de.ORCID http://orcid.org/0000-0002-0232-1903
Thorben CordesPhysical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians University München, Planegg-Martinsried, Germany. cordes@bio.lmu.de.ORCID http://orcid.org/0000-0002-8598-5499
Funding
Single Molecule Studies of Nucleic Acids RemodelingR35GM122569 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Taekjip Ha · 2017 to 2026
$3.5MStructural dynamics of RNAP-promoter complex in late transcription initiationR01GM130942 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WEISS, SHIMON · 2019 to 2022
$2.2MBiotechnology and Biological Sciences Research Council BB/T008032/1European Research Council 681891 (Prosint)European Research Council ERC-StG 638536-SM-IMPORTEuropean Research Council ERXC ADG MultiorganelldesignEuropean Research Council grant No. 671208 (hybridFRET)Marie Curie 101029907NIGMS NIH HHS R01 GM130942NIGMS NIH HHS R35 GM122569NIH HHS GM122569Wellcome TrustWellcome Trust 110164/Z/15/Z
Abstract
Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study of biomolecular structure and dynamics in vitro and in vivo. We performed an international blind study involving 19 laboratories to assess the uncertainty of FRET experiments for proteins with respect to the measured FRET efficiency histograms, determination of distances, and the detection and quantification of structural dynamics. Using two protein systems with distinct conformational changes and dynamics, we obtained an uncertainty of the FRET efficiency ≤0.06, corresponding to an interdye distance precision of ≤2 Å and accuracy of ≤5 Å. We further discuss the limits for detecting fluctuations in this distance range and how to identify dye perturbations. Our work demonstrates the ability of smFRET experiments to simultaneously measure distances and avoid the averaging of conformational dynamics for realistic protein systems, highlighting its importance in the expanding toolbox of integrative structural biology.
Indexed as
Fluorescence Resonance Energy TransferProteinsLaboratoriesMolecular ConformationReproducibility of ResultsProteins
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