Evidence map›Paper›PMID 36976029›Full record

ArticlemBio2023

Tuberculostearic Acid Controls Mycobacterial Membrane Compartmentalization.

Malavika Prithviraj, Takehiro Kado, Jacob A Mayfield, David C Young, Annie D Huang, Daisuke Motooka, Shota Nakamura, M Sloan Siegrist, D Branch Moody, Yasu S Morita

Open access · goldAbstract read
In one paragraph

Article in mBio, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Journal of bacteriology · 2026
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  6. Loss ofbioRxiv : the preprint server for biology · 2025
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  7. Effective killing ofMicrobiology spectrum · 2025
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  10. Acylation of glycerolipids in mycobacteria.Nature communications · 2023
    Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Malavika Prithviraj *Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.
Takehiro Kado *Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.
Jacob A Mayfield *Division of Rheumatology, Inflammation and Immunity, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
David C YoungDivision of Rheumatology, Inflammation and Immunity, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Annie D HuangDivision of Rheumatology, Inflammation and Immunity, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Daisuke MotookaResearch Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
Shota NakamuraResearch Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
M Sloan SiegristDepartment of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.ORCID 0000-0002-8232-3246
D Branch MoodyDivision of Rheumatology, Inflammation and Immunity, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Yasu S MoritaDepartment of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA.ORCID 0000-0002-4514-9242
Brigham and Women's Hospital · USUniversity of Massachusetts Amherst · USThe University of Osaka · JP

Funding

Study of M. tuberculosis under human host selection to identify virulence and barrier lipids (Project 1)U19AI162584 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI WOOD, ROBIN · 2021 to 2025
$13.5M
T cell response to CDI-restricted lipids in tuberculosisR01AI049313 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI MOODY, DAVID BRANCH · 2001 to 2025
$10.6M
CHEMISTRY-BIOLOGY INTERFACE PREDOCTORAL TRAINING GRANTST32GM008515 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI THOMPSON, LYNMARIE K. · 1995 to 2020
$4.3M
Chemistry-Biology Interface Predoctoral Training GrantT32GM139789 · NIGMS · UNIVERSITY OF MASSACHUSETTS AMHERST · PI ERIC Robert STRIETER · 2021 to 2026
$3.4M
Chemical Biological Discovery of Lipid Virulence Factors in the Major Bacterial PathogensR01AI165573 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI MOODY, DAVID BRANCH · 2022 to 2025
$3.0M
Spatial organization of mycobacterial cell wall biosynthesisR21AI144748 · NIAID · UNIVERSITY OF MASSACHUSETTS AMHERST · PI MORITA, YASU S, SIEGRIST, MARY SLOAN · 2019 to 2020
$423k
NIAID NIH HHS R01 AI049313NIAID NIH HHS R01 AI165573NIAID NIH HHS R21 AI144748NIAID NIH HHS U19 AI162584NIGMS NIH HHS T32 GM008515NIGMS NIH HHS T32 GM139789
6 · The paper itself

Abstract

The intracellular membrane domain (IMD) is a laterally discrete region of the mycobacterial plasma membrane, enriched in the subpolar region of the rod-shaped cell. Here, we report genome-wide transposon sequencing to discover the controllers of membrane compartmentalization in Mycobacterium smegmatis. The putative gene

Indexed as

DibucaineMycobacteriumFatty AcidsHumansMethyltransferasesOleic AcidStearic AcidsDibucaineFatty AcidsMethyltransferasesOleic AcidStearic Acidstuberculostearic acidfatty acidslipidomicsmembrane domainphospholipidstransposon sequencing

Identifiers

PMID36976029
PMCPMC10127668
OpenAlexW4361190630

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.