Evidence map›Paper›PMID 36977236›Full record

ArticleVeterinary sciences2023

Application of Multiparametric Flow Cytometry Panels to Study Lymphocyte Subpopulations in Tuberculin-Positive Cattle.

Anabelle Manzo-Sandoval, Laura Jaramillo-Meza, Roxana Olguín-Alor, Luvia Enid Sánchez-Torres, Fernando Díaz-Otero

Open access · goldAbstract read
In one paragraph

Article in Veterinary sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Anabelle Manzo-SandovalLaboratorio de Inmunología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal México-Toluca Km 15.5, Ciudad de México 05110, Mexico.ORCID 0000-0003-4997-8256
Laura Jaramillo-MezaLaboratorio de Inmunología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal México-Toluca Km 15.5, Ciudad de México 05110, Mexico.
Roxana Olguín-AlorLaboratorio Nacional de Citometría de Flujo, Instituto de Investigaciones Biomédicas, Universidad Autónoma de México, Circuito Escolar 33, Ciudad de México 04510, Mexico.
Luvia Enid Sánchez-TorresDepartamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala, s/n, Ciudad de México 11340, Mexico.ORCID 0000-0002-7128-849X
Fernando Díaz-OteroLaboratorio de Inmunología, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal México-Toluca Km 15.5, Ciudad de México 05110, Mexico.
Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias · MXInstituto Politécnico Nacional · MXUniversidad Nacional Autónoma de México · MX

Funding

Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias 142 945 340 13
6 · The paper itself

Abstract

Flow cytometry (FC) is widely used in microbiology, immunology, hematology, and oncology. In the veterinary field, FC enabled the study of the immune response in cattle infected with different pathogens, as well as vaccine testing. However, few fluorochrome-conjugated antibodies recognize bovine antigens, limiting the possible benefits of FC and the implementation of multiparametric analysis for more complex studies. Two cytometry panels with five colors each were designed and implemented for the study and identification of populations and subpopulations of T cells derived from the peripheral blood mononuclear cells of dairy heifers. Both panels detected differences in T cell subpopulations between heifers positively and negatively tested for tuberculin; they detected overexpression of CD25

Indexed as

bovineflow cytometrylymphocytesmonoclonal antibodiesmultiparametric panelMycobacterium bovis

Identifiers

PMID36977236
PMCPMC10057637
OpenAlexW4323317400

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.