Evidence mapPaperPMID 36977691Full record

ArticleScientific reports2023

A low dose of RBD and TLR7/8 agonist displayed on influenza virosome particles protects rhesus macaque against SARS-CoV-2 challenge.

Gerrit Koopman, Mario Amacker, Toon Stegmann, Ernst J Verschoor, Babs E Verstrepen, Farien Bhoelan, Denzel Bemelman, Kinga P Böszörményi, Zahra Fagrouch, Gwendoline Kiemenyi-Kayere and 9 more

Open access · goldFull text read
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 2 institutions in 2 countries.

Gerrit KoopmanDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands. koopman@bprc.nl.
Mario AmackerMymetics SA, 4 Route de La Corniche, 1066, Epalinges, Switzerland.
Toon StegmannMymetics BV, JH Oortweg 21, 2333 CH, Leiden, The Netherlands.
Ernst J VerschoorDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Babs E VerstrepenDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Farien BhoelanMymetics BV, JH Oortweg 21, 2333 CH, Leiden, The Netherlands.
Denzel BemelmanMymetics BV, JH Oortweg 21, 2333 CH, Leiden, The Netherlands.
Kinga P BöszörményiDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Zahra FagrouchDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Gwendoline Kiemenyi-KayereDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Daniella MortierDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Dagmar E VerelDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Henk NiphuisDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Roja Fidel AcarDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Ivanela KondovaAnimal Science Department, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Yolanda S KapDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Willy M J M BogersDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Petra MooijDepartment of Virology, Biomedical Primate Research Centre (BPRC), Rijswijk, The Netherlands.
Sylvain FleuryMymetics SA, 4 Route de La Corniche, 1066, Epalinges, Switzerland. sylvain.fleury@mymetics.com.
Biomedical Primate Research Centre · NLUniversity of Bern · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Influenza virosomes serve as antigen delivery vehicles and pre-existing immunity toward influenza improves the immune responses toward antigens. Here, vaccine efficacy was evaluated in non-human primates with a COVID-19 virosome-based vaccine containing a low dose of RBD protein (15 µg) and the adjuvant 3M-052 (1 µg), displayed together on virosomes. Vaccinated animals (n = 6) received two intramuscular administrations at week 0 and 4 and challenged with SARS-CoV-2 at week 8, together with unvaccinated control animals (n = 4). The vaccine was safe and well tolerated and serum RBD IgG antibodies were induced in all animals and in the nasal washes and bronchoalveolar lavages in the three youngest animals. All control animals became strongly sgRNA positive in BAL, while all vaccinated animals were protected, although the oldest vaccinated animal (V1) was transiently weakly positive. The three youngest animals had also no detectable sgRNA in nasal wash and throat. Cross-strain serum neutralizing antibodies toward Wuhan-like, Alpha, Beta, and Delta viruses were observed in animals with the highest serum titers. Pro-inflammatory cytokines IL-8, CXCL-10 and IL-6 were increased in BALs of infected control animals but not in vaccinated animals. Virosomes-RBD/3M-052 prevented severe SARS-CoV-2, as shown by a lower total lung inflammatory pathology score than control animals.

Indexed as

COVID-19Influenza, HumanInfluenza VaccinesAdjuvants, ImmunologicAnimalsAntibodies, NeutralizingAntibodies, ViralBroadly Neutralizing AntibodiesCOVID-19 VaccinesHumansMacaca mulattaSARS-CoV-2Toll-Like Receptor 7VirosomesAdjuvants, ImmunologicAntibodies, NeutralizingAntibodies, ViralBroadly Neutralizing AntibodiesCOVID-19 VaccinesInfluenza VaccinesToll-Like Receptor 7Virosomes

Identifiers

PMID36977691
PMCPMC10044094
OpenAlexW4361209697

What Socratic holds

Textfull text, public
LicenceCC BY
measurements read68
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.