Evidence map›Paper›PMID 37024962›Full record

ArticleBMC research notes2023

Particle bombardment-assisted peptide-mediated gene transfer for highly efficient transient assay.

Mitsuhiro Kimura, Akira Endo, Yozo Nagira, Takeshi Yoshizumi

Abstract read
In one paragraph

Article in BMC research notes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mitsuhiro KimuraFaculty of Agriculture, Takasaki University of Health and Welfare, Takasaki-shi, Gunma, Japan. mitsukimura@agr.kyushu-u.ac.jp.
Akira EndoAgri-Bio Research Center, Agri-Bio & Supplement Research Laboratories, Kaneka Corporation, Iwata, Shizuoka, Japan.
Yozo NagiraAgri-Bio Research Center, Agri-Bio & Supplement Research Laboratories, Kaneka Corporation, Iwata, Shizuoka, Japan.
Takeshi YoshizumiFaculty of Agriculture, Takasaki University of Health and Welfare, Takasaki-shi, Gunma, Japan. yoshidumi@takasaki-u.ac.jp.

Funding

Japan Society for the Promotion of Science 18K05638Japan Society for the Promotion of Science,Japan 20K21319
6 · The paper itself

Abstract

objectiveA centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for gene delivery into plant cells. However, the gene transfer efficiency of CAPT into plant cells was not entirely satisfactory for detecting transient expression of a transgene driven into mitochondria. Here, we report a new gene delivery system using a method called particle bombardment-assisted peptide-mediated gene transfer (PBPT).

resultsWe investigated various parameters of the PBPT method to increase transient gene expression efficiency in Brassica campestris. The optimal conditions for PBPT were a single bombardment with gold particles coated with a DNA‒peptide complex (6 µg of DNA and 2 µg of peptide) at an acceleration pressure of 5 kg/cm

Indexed as

DNAGene Transfer TechniquesPeptidesPlantsTransformation, GeneticDNAPeptidesDNA-peptide complexNanolucParticle bombardment-assisted peptide-mediated gene transfer (PBPT)

Identifiers

PMID37024962
PMCPMC10080836

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.