ArticleFrontiers in immunology2023
Orf virus DNA prime-protein boost strategy is superior to adenovirus-based vaccination in mice and sheep.
Article in Frontiers in immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 12 citations in OpenAlex.
- Host-Orf virus interactions: A comprehensive review of pathogenesis and immune evasion mechanisms.Virulence · 2026Review
- An ORFV F1L mRNA Vaccine Candidate: Preparation, Immunogenicity, and Comparison with a Commercial Live Vaccine.Animals : an open access journal from MDPI · 2026Article
- Molecular Characterisation of Orf Virus in Goats From Eastern Türkiye.Veterinary medicine and science · 2026Article
- Article
- The Treatment of Contagious Ecthyma in Lambs with a Local Anaesthetic/Antiseptic Wound Formulation Lowers Serum Amyloid A Responses.Animals : an open access journal from MDPI · 2025Article
- Enhanced antibody response to the conformational non-RBD regionEmerging microbes & infections · 2025Article
- Development of two indirect ELISAs using purified recombinant viral proteins to detect myxoma virus-antigen specific antibodies in rabbit sera.BMC veterinary research · 2025Article
- DNA vaccine targeting betacoronavirus spike protein blocks neuroinvasion and neuroinflammation in swine via dual antiviral-immunomodulatory action.NPJ vaccines · 2025Article
- Novel insights into ORFV B2L DNA vaccine-mediated gut microbiota modulation and immune augmentation in rats.Frontiers in immunology · 2025Article
- Designing a multi-epitope subunit vaccine against Orf virus using molecular docking and molecular dynamics.Virulence · 2024Article
- Novel Multi-Antigen Orf-Virus-Derived Vaccine Elicits Protective Anti-SARS-CoV-2 Response in Monovalent and Bivalent Formats.Vaccines · 2024Article
- Molecular detection and phylogenetic analysis of Orf viruses from goats in Jiangxi province, China.Frontiers in veterinary science · 2024Article
- Screening and characterization of a novel linear B-cell epitope on orf virus F1L protein.Frontiers in microbiology · 2024Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contagious ecthyma (Orf), an acute and highly contagious zoonosis, is prevalent worldwide. Orf is caused by Orf virus (ORFV), which mainly infects sheep/goats and humans. Therefore, effective and safe vaccination strategies for Orf prevention are needed. Although immunization with single-type Orf vaccines has been tested, heterologous prime-boost strategies still need to be studied. In the present study, ORFV B2L and F1L were selected as immunogens, based on which DNA, subunit and adenovirus vaccine candidates were generated. Of note, heterologous immunization strategies using DNA prime-protein boost and DNA prime-adenovirus boost in mice were performed, with single-type vaccines as controls. We have found that the DNA prime-protein boost strategy induces stronger humoral and cellular immune responses than DNA prime-adenovirus boost strategy in mice, which was confirmed by the changes in specific antibodies, lymphocyte proliferation and cytokine expression. Importantly, this observation was also confirmed when these heterologous immunization strategies were performed in sheep. In summary, by comparing the two immune strategies, we found that DNA prime-protein boost strategy can induce a better immune response, which provides a new attempt for exploring Orf immunization strategy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.