Evidence map›Paper›PMID 37029114›Full record

ArticleCell death & disease2023

Enhancer of zeste homolog 2 promotes renal fibrosis after acute kidney injury by inducing epithelial-mesenchymal transition and activation of M2 macrophage polarization.

Xun Zhou, Hui Chen, Yan Hu, Xiaoyan Ma, Jinqing Li, Yingfeng Shi, Min Tao, Yi Wang, Qin Zhong, Danying Yan and 2 more

Open access · goldAbstract read
In one paragraph

Article in Cell death & disease, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
11.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 66 citations in OpenAlex.

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  16. Epigenetic memories induced by hypoxia in AKI-to-CKD transition.Clinical and experimental nephrology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Xun Zhou *Department of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Hui Chen *Department of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Yan Hu *Department of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Xiaoyan MaDepartment of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Jinqing LiDepartment of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Yingfeng ShiDepartment of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Min TaoDepartment of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Yi WangDepartment of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Qin ZhongDepartment of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Danying YanDepartment of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Shougang ZhuangDepartment of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Na LiuDepartment of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China. naliubrown@163.com.ORCID 0000-0001-5806-8209
Shanghai East Hospital · CNBrown University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Long-term follow-up data indicates that 1/4 patients with acute kidney injury (AKI) will develop to chronic kidney disease (CKD). Our previous studies have demonstrated that enhancer of zeste homolog 2 (EZH2) played an important role in AKI and CKD. However, the role and mechanisms of EZH2 in AKI-to-CKD transition are still unclear. Here, we demonstrated EZH2 and H3K27me3 highly upregulated in kidney from patients with ANCA-associated glomerulonephritis, and expressed positively with fibrotic lesion and negatively with renal function. Conditional EZH2 deletion or pharmacological inhibition with 3-DZNeP significantly improved renal function and attenuated pathological lesion in ischemia/reperfusion (I/R) or folic acid (FA) mice models (two models of AKI-to-CKD transition). Mechanistically, we used CUT & Tag technology to verify that EZH2 binding to the PTEN promoter and regulating its transcription, thus regulating its downstream signaling pathways. Genetic or pharmacological depletion of EZH2 upregulated PTEN expression and suppressed the phosphorylation of EGFR and its downstream signaling ERK1/2 and STAT3, consequently alleviating the partial epithelial-mesenchymal transition (EMT), G2/M arrest, and the aberrant secretion of profibrogenic and proinflammatory factors in vivo and vitro experiments. In addition, EZH2 promoted the EMT program induced loss of renal tubular epithelial cell transporters (OAT1, ATPase, and AQP1), and blockade of EZH2 prevented it. We further co-cultured macrophages with the medium of human renal tubular epithelial cells treated with H

Indexed as

Acute Kidney InjuryEnhancer of Zeste Homolog 2 ProteinEpithelial-Mesenchymal TransitionMacrophagesRenal Insufficiency, ChronicAnimalsApoptosisCell Line, TumorFibrosisG2 Phase Cell Cycle CheckpointsHumansHydrogen PeroxideKidneyMicePhosphatidylinositol 3-KinasesEnhancer of Zeste Homolog 2 ProteinEZH2 protein, humanHydrogen PeroxidePhosphatidylinositol 3-Kinases

Identifiers

PMID37029114
PMCPMC10081989
OpenAlexW4362701606

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.