Evidence map›Paper›PMID 37035009›Full record

ArticleiScience2023

Methacrylated human recombinant collagen peptide as a hydrogel for manipulating and monitoring stiffness-related cardiac cell behavior.

Dylan Mostert, Ignasi Jorba, Bart G W Groenen, Robert Passier, Marie-José T H Goumans, Huibert A van Boxtel, Nicholas A Kurniawan, Carlijn V C Bouten, Leda Klouda

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Dylan MostertDepartment of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.
Ignasi JorbaDepartment of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.
Bart G W GroenenDepartment of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.
Robert PassierDepartment of Applied Stem Cell Technologies, University of Twente, 7522 NB Enschede, the Netherlands.
Marie-José T H GoumansDepartment of Cell and Chemical Biology and Center for Biomedical Genetics, Leiden University Medical Centre, 2333 ZA Leiden, the Netherlands.
Huibert A van BoxtelFujifilm Manufacturing Europe B.V., 5047 TK Tilburg, the Netherlands.
Nicholas A KurniawanDepartment of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.
Carlijn V C BoutenDepartment of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.
Leda KloudaDepartment of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, the Netherlands.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Environmental stiffness is a crucial determinant of cell function. There is a long-standing quest for reproducible and (human matrix) bio-mimicking biomaterials with controllable mechanical properties to unravel the relationship between stiffness and cell behavior. Here, we evaluate methacrylated human recombinant collagen peptide (RCPhC1-MA) hydrogels as a matrix to control 3D microenvironmental stiffness and monitor cardiac cell response. We show that RCPhC1-MA can form hydrogels with reproducible stiffness in the range of human developmental and adult myocardium. Cardiomyocytes (hPSC-CMs) and cardiac fibroblasts (cFBs) remain viable for up to 14 days inside RCPhC1-MA hydrogels while the effect of hydrogel stiffness on extracellular matrix production and hPSC-CM contractility can be monitored in real-time. Interestingly, whereas the beating behavior of the hPSC-CM monocultures is affected by environmental stiffness, this effect ceases when cFBs are present. Together, we demonstrate RCPhC1-MA to be a promising candidate to mimic and control the 3D biomechanical environment of cardiac cells.

Indexed as

BiomaterialsCell biologyMaterials in biotechnologyStem cells research

Identifiers

PMID37035009
PMCPMC10074202

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.