Evidence map›Paper›PMID 37035329›Full record

ArticleFrontiers in medicine2023

Spatial distribution of CD3- and CD8-positive lymphocytes as pretest for POLE wild-type in molecular subgroups of endometrial carcinoma.

Samuel H Jungen, Luca Noti, Lucine Christe, Jose A Galvan, Inti Zlobec, Michael D Müller, Sara Imboden, Franziska Siegenthaler, Joseph W Carlson, Teijo Pellinen and 6 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 6 countries.

Samuel H JungenInstitute of Pathology, University of Bern, Bern, Switzerland.
Luca NotiInstitute of Pathology, University of Bern, Bern, Switzerland.
Lucine ChristeInstitute of Pathology, University of Bern, Bern, Switzerland.
Jose A GalvanInstitute of Pathology, University of Bern, Bern, Switzerland.
Inti ZlobecInstitute of Pathology, University of Bern, Bern, Switzerland.
Michael D MüllerDepartment of Obstetrics and Gynecology, University Hospital of Bern, University of Bern, Bern, Switzerland.
Sara ImbodenDepartment of Obstetrics and Gynecology, University Hospital of Bern, University of Bern, Bern, Switzerland.
Franziska SiegenthalerDepartment of Obstetrics and Gynecology, University Hospital of Bern, University of Bern, Bern, Switzerland.
Joseph W CarlsonKarolinska Institutet, Klinisk Patologi KS, Solna, Sweden.
Teijo PellinenInstitute for Molecular Medicine Finland, Helsinki, Finland.
Victoria Heredia-SotoInstituto de Investigación Biomédica del Hospital Universitario La Paz (IdiPAZ), Madrid, Spain.
Ignacio Ruz-CaracuelDepartment of Pathology, Hospital Universitario La Paz, Madrid, Spain.
David HardissonInstituto de Investigación Biomédica del Hospital Universitario La Paz (IdiPAZ), Madrid, Spain.
Andres RedondoDepartment of Medical Oncology, Hospital Universitario La Paz, Madrid, Spain.
Marta MendiolaInstituto de Investigación Biomédica del Hospital Universitario La Paz (IdiPAZ), Madrid, Spain.
Tilman T RauInstitute of Pathology, University of Bern, Bern, Switzerland.
University of Bern · CHHospital Universitario La Paz · ESInstitute for Molecular Medicine Finland · FIKeck Hospital of USC · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Over the years, the molecular classification of endometrial carcinoma has evolved significantly. Both POLEmut and MMRdef cases share tumor biological similarities like high tumor mutational burden and induce strong lymphatic reactions. While therefore use case scenarios for pretesting with tumor-infiltrating lymphocytes to replace molecular analysis did not show promising results, such testing may be warranted in cases where an inverse prediction, such as that of POLEwt, is being considered. For that reason we used a spatial digital pathology method to quantitatively examine CD3 Methods: We applied a four-color multiplex immunofluorescence assay for pan-cytokeratin, CD3, CD8, and DAPI on 252 endometrial carcinomas as testing and compared it to further 213 cases as validation cohort from a similar multiplexing assay. We quantitatively assessed immune infiltrates in microscopic distances within the carcinoma, in a close distance of 50 microns, and in more distant areas. Results: Regarding prognostics, high CD3 Discussion: Molecular confirmation of POLEmut cases remains the gold standard. Even if CD3

Indexed as

CD3CD8endometrial carcinomamultiplex immunofluorescencePOLETCGA

Identifiers

PMID37035329
PMCPMC10076655
OpenAlexW4360618930

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.