Evidence map›Paper›PMID 37046285›Full record

ArticleJournal of ovarian research2023

Androgen-induced exosomal miR-379-5p release determines granulosa cell fate: cellular mechanism involved in polycystic ovaries.

Reza Salehi, Brandon A Wyse, Meshach Asare-Werehene, Fereshteh Esfandiarinezhad, Atefeh Abedini, Bo Pan, Yoko Urata, Alex Gutsol, Jose L Vinas, Sahar Jahangiri and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 8 institutions in 3 countries.

Reza SalehiChronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Brandon A WyseCReATe Fertility Centre, Toronto, ON, Canada.
Meshach Asare-WereheneChronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Fereshteh EsfandiarinezhadChronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Atefeh AbediniCancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Bo PanDepartment of Animal BioScience, University of Guelph, Guelph, ON, Canada.
Yoko UrataChronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Alex GutsolDivision of Nephrology, Department of Medicine, Kidney Research Centre, University of Ottawa, Ottawa, ON, Canada.
Jose L VinasDivision of Nephrology, Department of Medicine, Kidney Research Centre, University of Ottawa, Ottawa, ON, Canada.
Sahar JahangiriCReATe Fertility Centre, Toronto, ON, Canada.
Kai XueDepartment of Gynecology, The Affiliated Obstetrics and Gynecology Hospital, Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu Province, China.
Yunping XueChronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Kevin D BurnsDivision of Nephrology, Department of Medicine, Kidney Research Centre, University of Ottawa, Ottawa, ON, Canada.
Barbara VanderhydenDepartments of Obstetrics and Gynecology, and Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Julang LiDepartment of Animal BioScience, University of Guelph, Guelph, ON, Canada.
Yutaka OsugaDepartment of Obstetrics and Gynecology, University of Tokyo, Tokyo, Japan.
Dylan BurgerChronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Seang-Lin TanDepartment of Obstetrics and Gynecology, McGill University, Montreal, QC, Canada.
Clifford L LibrachCReATe Fertility Centre, Toronto, ON, Canada.
Benjamin K TsangChronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada. btsang@ohri.ca.
Ottawa Hospital Research InstituteCReATe Fertility Centre · CAOttawa Hospital · CAUniversity of Ottawa · CAUniversity of Guelph · CAMcGill University · CANanjing Maternity and Child Health Care Hospital · CNThe University of Tokyo · JP

Funding

CIHR MOP-119381
6 · The paper itself

Abstract

Polycystic ovarian syndrome (PCOS) is a complex multi-factorial syndrome associated with androgen excess and anovulatory infertility. In the current study, we investigated the role of dihydrotestosterone-induced exosomal miR-379-5p release in determining the destiny of the developing follicles. Our hypothesis was that androgen regulates granulosa cell miR-379-5p content by facilitating its exosomal release in a follicular-stage dependent manner, a process which determines granulosa cell fate. Compared to human non-PCOS subjects, individuals with PCOS exhibit higher follicular fluid free testosterone levels, lower exosomal miR-379-5p content and granulosa cell proliferation. Androgenized rats exhibited lower granulosa cell miR-379-5p but higher phosphoinositide-dependent kinase-1 (PDK1; a miR-379-5p target) content and proliferation. Androgen reduced granulosa cell miR-379-5p content by increasing its exosomal release in preantral follicles, but not in antral follicles in vitro. Studies with an exosomal release inhibitor confirmed that androgen-induced exosomal miR-379-5p release decreased granulosa cell miR-379-5p content and proliferation. Ovarian overexpression of miR-379-5p suppressed granulosa cell proliferation, and basal and androgen-induced preantral follicle growth in vivo. These findings suggest that increased exosomal miR-379-5p release in granulosa cells is a proliferative response to androgenic stimulation specific for the preantral stage of follicle development and that dysregulation of this response at the antral stage is associated with follicular growth arrest, as observed in human PCOS.

Indexed as

MicroRNAsPolycystic Ovary SyndromeAndrogensAnimalsFemaleGranulosa CellsHumansRatsAndrogensMicroRNAsMIRN379 microRNA, human

Identifiers

PMID37046285
PMCPMC10091561
OpenAlexW4365150143

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.