Evidence mapPaperPMID 37147336Full record

ArticleNature communications2023

Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus.

Pengcheng Shang, Joshua D Simpson, Gwen M Taylor, Danica M Sutherland, Olivia L Welsh, Pavithra Aravamudhan, Rita Dos Santos Natividade, Kristina Schwab, Joshua J Michel, Amanda C Poholek and 5 more

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
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  16. Immune receptors and aging brain.Bioscience reports · 2024
    Review
  17. Article
  18. NgR1 binding to reovirus reveals an unusual bivalent interaction and a new viral attachment protein.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 3 countries.

Pengcheng ShangDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.ORCID 0000-0002-6174-2353
Joshua D SimpsonLouvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.ORCID 0000-0002-8823-8130
Gwen M TaylorDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.ORCID 0000-0002-9276-087X
Danica M SutherlandDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.ORCID 0000-0001-9328-4994
Olivia L WelshDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Pavithra AravamudhanDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Rita Dos Santos NatividadeLouvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.ORCID 0000-0002-0123-4760
Kristina SchwabDepartment of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Joshua J MichelDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Amanda C PoholekDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.ORCID 0000-0002-5587-8940
Yijen WuDepartment of Developmental Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.ORCID 0000-0001-9509-5417
Dhivyaa RajasundaramDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Melanie KoehlerLeibniz Institute for Food Systems Biology at the Technical University Munich, Freising, Germany.
David AlsteensLouvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, Louvain-la-Neuve, Belgium.ORCID 0000-0001-9229-113X
Terence S DermodyDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. terence.dermody@chp.edu.ORCID 0000-0003-1853-8741
University of Pittsburgh · USUCLouvain · BELeibniz-Institute for Food Systems Biology at the Technical University of Munich · DEWalloon Excellence in Lifesciences and Biotechnology · BE

Funding

Reovirus NeuropathogenesisR01AI174526 · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2025 to 2025
$520k
NIAID NIH HHS R01 AI174526NIH HHS S10 OD028483
6 · The paper itself

Abstract

Mammalian orthoreovirus (reovirus) infects most mammals and is associated with celiac disease in humans. In mice, reovirus infects the intestine and disseminates systemically to cause serotype-specific patterns of disease in the brain. To identify receptors conferring reovirus serotype-dependent neuropathogenesis, we conducted a genome-wide CRISPRa screen and identified paired immunoglobulin-like receptor B (PirB) as a receptor candidate. Ectopic expression of PirB allowed reovirus binding and infection. PirB extracelluar D3D4 region is required for reovirus attachment and infectivity. Reovirus binds to PirB with nM affinity as determined by single molecule force spectroscopy. Efficient reovirus endocytosis requires PirB signaling motifs. In inoculated mice, PirB is required for maximal replication in the brain and full neuropathogenicity of neurotropic serotype 3 (T3) reovirus. In primary cortical neurons, PirB expression contributes to T3 reovirus infectivity. Thus, PirB is an entry receptor for reovirus and contributes to T3 reovirus replication and pathogenesis in the murine brain.

Indexed as

Orthoreovirus, MammalianReceptors, ImmunologicReceptors, VirusReoviridae InfectionsAnimalsAntibodies, ViralHumansMiceAntibodies, ViralPirb protein, mouseReceptors, ImmunologicReceptors, Virus

Identifiers

PMID37147336
PMCPMC10163058
OpenAlexW4372279043

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.