Evidence map›Paper›PMID 37163022›Full record

ArticlebioRxiv : the preprint server for biology2023

High-throughput optical sensing of peri-cellular oxygen in cardiac cells: system characterization, calibration, and testing.

Weizhen Li, David McLeod, John T Ketzenberger, Grant Kowalik, Rebekah Russo, Zhenyu Li, Matthew W Kay, Emilia Entcheva

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Weizhen LiDepartment of Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052.
David McLeodDepartment of Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052.
John T KetzenbergerDepartment of Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052.
Grant KowalikDepartment of Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052.
Rebekah RussoDepartment of Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052.
Zhenyu LiDepartment of Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052.
Matthew W KayDepartment of Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052.
Emilia EntchevaDepartment of Biomedical Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC 20052.
George Washington University · US

Funding

Scalable platform for optimizing human cardiac tissue engineering via optical pacing and on-demand oxygenationR01HL144157 · NHLBI · GEORGE WASHINGTON UNIVERSITY · PI ENTCHEVA, EMILIA, KAY, MATTHEW W. · 2019 to 2022
$2.8M
NHLBI NIH HHS R01 HL144157
6 · The paper itself

Abstract

Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) represent a scalable experimental model relevant to human physiology. Oxygen consumption of hiPSC-CMs has not been studied in high-throughput (HT) format plates used in pre-clinical studies. Here, we provide comprehensive characterization and validation of a system for HT long-term optical measurements of peri-cellular oxygen in cardiac syncytia (human iPSC-CM and human cardiac fibroblasts), grown in glass-bottom 96-well plates. Laser-cut oxygen sensors having a ruthenium dye and an oxygen-insensitive reference dye were used. Ratiometric measurements (409nm excitation) reflected dynamic changes in oxygen, as validated with simultaneous Clark electrode measurements. Emission ratios (653nm vs. 510nm) were calibrated for percent oxygen using two-point calibration. Time-dependent changes in the Stern-Volmer parameter, Ksv, were observed during the initial 40 min of incubation, likely temperature-related. Effects of pH on oxygen measurements were negligible in the pH range of 4 to 8, with a small ratio reduction for pH>10. Time-dependent calibration was implemented, and light exposure time was optimized (0.6 to 0.8s) for oxygen measurements inside an incubator. Peri-cellular oxygen dropped to levels < 5% within 3 -10 hours for densely-plated hiPSC-CMs in glass-bottom 96-well plates. After the initial oxygen decrease, samples either settled to low steady-state or exhibited intermittent peri-cellular oxygen dynamics. Cardiac fibroblasts showed slower oxygen depletion and higher steady-state levels without oscillations, compared to hiPSC-CMs. Overall, the system has great utility for long-term HT monitoring of peri-cellular oxygen dynamics in vitro for tracking cellular oxygen consumption, metabolic perturbations, and characterization of the maturation of hiPSC-CMs.

Identifiers

PMID37163022
PMCPMC10168216
OpenAlexW4367296175

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.