ArticleBMC genomics2023
Identification and validation of the reference genes in the echiuran worm Urechis unicinctus based on transcriptome data.
Article in BMC genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed, 20 citations in OpenAlex.
- Transcriptomic analysis reveals functional compartmentalization of the digestive tract in Urechis unicinctus.Genes & genomics · 2026Article
- Identification and validation of stable reference genes via transcriptome analysis for studying sex differentiation in tung tree (Vernicia fordii).BMC genomics · 2026Article
- Validation of reference genes for RT-qPCR relative expression analysis during cyst-to-early adult development of Taenia solium.PLoS neglected tropical diseases · 2026Article
- Identification and validation of reference genes for qPCR of Pseudomonas aeruginosa L10 under varying n-hexadecane concentrations.BMC microbiology · 2025Article
- Screening and validation of optimal real-time PCR reference genes for Abelmoschus Manihot.Scientific reports · 2025Article
- Identification and validation of stable reference genes for RT-qPCR analyses of Kobresia littledalei seedlings.BMC plant biology · 2024Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundReal-time quantitative PCR (RT-qPCR) is a crucial and widely used method for gene expression analysis. Selecting suitable reference genes is extremely important for the accuracy of RT-qPCR results. Commonly used reference genes are not always stable in various organisms or under different environmental conditions. With the increasing application of high-throughput sequencing, transcriptome analysis has become an effective method for identifying novel stable reference genes.
resultsIn this study, we identified candidate reference genes based on transcriptome data covering embryos and larvae of early development, normal adult tissues, and the hindgut under sulfide stress using the coefficient of variation (CV) method in the echiuran Urechis unicinctus, resulting in 6834 (15.82%), 7110 (16.85%) and 13880 (35.87%) candidate reference genes, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the candidate reference genes were significantly enriched in cellular metabolic process, protein metabolic process and ribosome in early development and normal adult tissues as well as in cellular localization and endocytosis in the hindgut under sulfide stress. Subsequently, ten genes including five new candidate reference genes and five commonly used reference genes, were validated by RT-qPCR. The expression stability of the ten genes was analyzed using four methods (geNorm, NormFinder, BestKeeper, and ∆Ct). The comprehensive results indicated that the new candidate reference genes were more stable than most commonly used reference genes. The commonly used ACTB was the most unstable gene. The candidate reference genes STX12, EHMT1, and LYAG were the most stable genes in early development, normal adult tissues, and hindgut under sulfide stress, respectively. The log
conclusionOur study is the first to select reference genes for RT-qPCR from transcriptome data in Echiura and provides important information for future gene expression studies in U. unicinctus.
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