Evidence map›Paper›PMID 37168944›Full record

ArticleAmerican journal of clinical and experimental urology2023

Novel urinary tract obstruction marker discovery by multi-marker profiling of urinary extracellular vesicles derived from a rat UTO model.

Nora M Haney, Chi-Ju Kim, Morgan D Kuczler, Cheng-Fan Lee, Kara Lombardo, Trinity J Bivalacqua, Kenneth J Pienta, Sarah R Amend

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in American journal of clinical and experimental urology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Nora M HaneyThe Brady Urological Institute, Johns Hopkins School of Medicine 600 N. Wolfe St., Baltimore, MD 21287, USA.
Chi-Ju KimCancer Ecology Center, The Brady Urological Institute, Johns Hopkins School of Medicine 600 N. Wolfe St., Baltimore, MD 21287, USA.
Morgan D KuczlerCancer Ecology Center, The Brady Urological Institute, Johns Hopkins School of Medicine 600 N. Wolfe St., Baltimore, MD 21287, USA.
Cheng-Fan LeeCancer Ecology Center, The Brady Urological Institute, Johns Hopkins School of Medicine 600 N. Wolfe St., Baltimore, MD 21287, USA.
Kara LombardoThe Brady Urological Institute, Johns Hopkins School of Medicine 600 N. Wolfe St., Baltimore, MD 21287, USA.
Trinity J BivalacquaDivision of Urology, University of Pennsylvania Perelman School of Medicine 3400 Civic Center Boulevard West Pavilion, Philadelphia, PA 19104, USA.
Kenneth J PientaCancer Ecology Center, The Brady Urological Institute, Johns Hopkins School of Medicine 600 N. Wolfe St., Baltimore, MD 21287, USA.
Sarah R AmendCancer Ecology Center, The Brady Urological Institute, Johns Hopkins School of Medicine 600 N. Wolfe St., Baltimore, MD 21287, USA.
Johns Hopkins University · USUniversity of Pennsylvania · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionCongenital urinary obstruction is a common cause of end-stage renal disease in the pediatric population. However, non-invasive diagnostics to predict which patients will benefit from early intervention are lacking.

methodsUsing a rat model of upper and lower urinary tract partial obstruction and the Nanostring nCounter Fibrosis V2 Panel, we evaluated the mRNA cargo of urinary small extracellular vesicles (sEVs) and mRNA expression patterns of kidney and bladder tissues from rats with lower tract urinary obstruction and upper tract urinary obstruction.

resultsWhile mRNA hierarchical clustering of urinary sEVs was unable to differentiate upper compared to lower tract urinary obstruction, clustering was able to detect overall disease state (UUTO or LUTO) versus healthy controls. Further, urinary sEVs carried genes unique to each treatment group (UUTO: 59 genes, LUTO: 17 genes), while only one gene was uniquely carried in the control group. Notable genes of interest found in urinary sEVs were VCAM-1 and NOS1 for UUTO, Egfr for LUTO, and Pck1 for healthy controls.

conclusionThis study provides support that differential gene expression of urinary sEV mRNA has potential to act as biomarkers in the diagnosis and prognosis of UTO. Urinary sEVs demonstrated higher numbers of unique genes representative of injury to the kidney than that of injury to the bladder. Importantly, there were genes unique to UUTO sEVs, indicating the extent and reversibility of renal damage can be independent of the function, damage, and architecture of the bladder.

Indexed as

extracellular vesiclesurinary biomarkerUrinary tract obstruction

Identifiers

PMID37168944
PMCPMC10165232
OpenAlexW4376223737

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.