Evidence map›Paper›PMID 37171000›Full record

ArticleCombinatorial chemistry & high throughput screening2024

A Stable Cell Line Co-expressing hTRPV1 and GCaMP6s: A Novel Cell-based Assay For High-throughput Screening of hTRPV1 Agonists.

Jingjing Shi, Xuejun Chen, Yi Zhang, Tong Shi, Ruihua Zhang, Siqing Zhu, Xingxing Zong, Chen Wang, Liqin Li

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Article in Combinatorial chemistry & high throughput screening, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jingjing ShiDepartment of Laboratory of Pharmacology, State Key Laboratory of NBC Protection for Civilians, Beijing, 102205, China.
Xuejun ChenDepartment of Laboratory of Pharmacology, State Key Laboratory of NBC Protection for Civilians, Beijing, 102205, China.
Yi ZhangDepartment of Laboratory of Pharmacology, State Key Laboratory of NBC Protection for Civilians, Beijing, 102205, China.
Tong ShiDepartment of Laboratory of Pharmacology, State Key Laboratory of NBC Protection for Civilians, Beijing, 102205, China.
Ruihua ZhangDepartment of Laboratory of Pharmacology, State Key Laboratory of NBC Protection for Civilians, Beijing, 102205, China.
Siqing ZhuDepartment of Laboratory of Pharmacology, State Key Laboratory of NBC Protection for Civilians, Beijing, 102205, China.
Xingxing ZongDepartment of Laboratory of Pharmacology, State Key Laboratory of NBC Protection for Civilians, Beijing, 102205, China.
Chen WangDepartment of Laboratory of Pharmacology, State Key Laboratory of NBC Protection for Civilians, Beijing, 102205, China.
Liqin LiDepartment of Laboratory of Pharmacology, State Key Laboratory of NBC Protection for Civilians, Beijing, 102205, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTransient receptor potential vanilloid-1 (TRPV1) is a non-selective cation channel capable of integrating various noxious chemical and physical stimuli. Recently, human TRPV1 (hTRPV1) has attracted wide attention from researchers because it is closely related to pain, inflammation, temperature perception, and tumors. Our study was aimed at generating a stable cell line co-expressing hTRPV1 receptor and GCaMP6s calcium indicator protein and, based on this, developing high-throughput screening methods for targeting hTRPV1 agonists.

methodsThe CHO-hTRPV1-GCaMP6s cell line stably expressing hTRPV1 and GCaMP6s was generated by co-transfection of hTRPV1 and GCaMP6s into Chinese hamster ovary (CHO) cells. The high-throughput screening methods were developed based on detecting the concentration of intracellular calcium ions ([Ca

resultsA stable cell line co-expressing hTRPV1 and GCaMP6s was generated and used to establish a functional high-throughput screening assay based on the measurement of [Ca

conclusionThe successful establishment of the CHO-hTRPV1-GCaMP6s cell lines and their application in high-throughput screening of hTRPV1 agonists.

Indexed as

CalciumCricetulusHigh-Throughput Screening AssaysTRPV Cation ChannelsAnimalsCapsaicinCHO CellsCricetinaeHumansCalciumCapsaicinTRPV1 protein, humanTRPV Cation ChannelsCalciumcapsaicinfluorometric imaging plate readerGCaMP6shigh-throughput screeninghuman transient receptor potential vanilloid-1.

Identifiers

PMID37171000
OpenAlexW4376224025

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.