Evidence map›Paper›PMID 37175035›Full record

ArticleDiagnostics (Basel, Switzerland)2023

Application of Hybridization Chain Reaction/CRISPR-Cas12a for the Detection of SARS-CoV-2 Infection.

Kate Obaayaa Sagoe, Mutinda Cleophas Kyama, Naomi Maina, Moses Kamita, Muturi Njokah, Kelvin Thiong'o, Bernard N Kanoi, Ernest Apondi Wandera, Davies Ndegwa, Dickson Mwenda Kinyua and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostics (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Kate Obaayaa SagoeDepartment of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation (PAUSTI), Nairobi P.O. Box 62000-00200, Kenya.ORCID 0000-0002-1615-6662
Mutinda Cleophas KyamaDepartment of Medical Laboratory Science, College of Health Sciences, Jomo Kenyatta University of Agriculture & Technology, Nairobi P.O. Box 62000-00200, Kenya.
Naomi MainaDepartment of Biochemistry, College of Health Sciences, Jomo Kenyatta University of Agriculture & Technology, Nairobi P.O. Box 62000-00200, Kenya.
Moses KamitaDirectorate of Research and Innovation, Mount Kenya University, Thika P.O. Box 342-01000, Kenya.ORCID 0000-0002-1756-932X
Muturi NjokahDepartment of Biochemistry, College of Health Sciences, Jomo Kenyatta University of Agriculture & Technology, Nairobi P.O. Box 62000-00200, Kenya.
Kelvin Thiong'oCenter for Biotechnology Research and Development, Kenya Medical Research Institute, Nairobi P.O. Box 54840-00200, Kenya.
Bernard N KanoiDirectorate of Research and Innovation, Mount Kenya University, Thika P.O. Box 342-01000, Kenya.ORCID 0000-0003-2772-7579
Ernest Apondi WanderaDirectorate of Research and Innovation, Mount Kenya University, Thika P.O. Box 342-01000, Kenya.
Davies NdegwaDepartment of Medical Laboratory Sciences, Kenya Medical Training College, Nairobi P.O. Box 30195-00100, Kenya.
Dickson Mwenda KinyuaDepartment of Physical Sciences, Meru University of Science & Technology, Meru P.O. Box 972-60200, Kenya.
Jesse GitakaDirectorate of Research and Innovation, Mount Kenya University, Thika P.O. Box 342-01000, Kenya.

Funding

National Research Fund KenyaPan African University Institute for Basic Sciences, Technology and Innovation PAU/ADM/PAUSTI/2020/8
6 · The paper itself

Abstract

Globally, the emergence of the coronavirus disease (COVID-19) has had a significant impact on life. The need for ongoing SARS-CoV-2 screening employing inexpensive and quick diagnostic approaches is undeniable, given the ongoing pandemic and variations in vaccine administration in resource-constrained regions. This study presents results as proof of concept to use hybridization chain reaction (HCR) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a complex for detecting SARS-CoV-2. HCR hairpin probes were designed using the NUPACK web-based program and further used to amplify the SARS-CoV-2 N gene in archived nasopharyngeal samples. The results were visualized using agarose gels and CRISPR Cas12a-based lateral flow strips. The assay was evaluated using the gold standard, real-time polymerase chain reaction (RT-PCR), as recommended by the World Health Organization (WHO). The results show the comparative efficiency of HCR to RT-PCR. This study shows that HCR and CRISPR are viable alternatives for diagnosing SARS-CoV-2 in samples.

Indexed as

clustered regularly interspaced short palindromic repeats (CRISPR)COVID-19diagnosticshybridization chain reaction (HCR)point-of-care (POC)severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Identifiers

PMID37175035
PMCPMC10178590

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.