Evidence mapPaperPMID 37189439Full record

ReviewBiomolecules2023

Multiomics Analysis Reveals Novel Genetic Determinants for Lens Differentiation, Structure, and Transparency.

Joshua Disatham, Lisa Brennan, Ales Cvekl, Marc Kantorow

Abstract readReview
In one paragraph

Review in Biomolecules, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Joshua DisathamCharles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL 33431, USA.
Lisa BrennanCharles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL 33431, USA.
Ales CveklDepartments of Ophthalmology and Visual Sciences and Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.ORCID 0000-0002-3957-789X
Marc KantorowCharles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL 33431, USA.

Funding

PAX-6 AS A KEY REGULATOR OF LENS DEVELOPMENTR01EY012200 · YESHIVA UNIVERSITY · 2000 to 2025
$3.0M
Transcriptional control of the Alpha A-crystallin locusR01EY014237 · YESHIVA UNIVERSITY · 2003 to 2005
$1.1M
NEI NIH HHS R01 EY012200NEI NIH HHS R01 EY014237NIH HHS R01 EY029708 (MK), R01 EY026478 (MK, ASM), R01 EY012200, EY014237 and EY014237-19S1 (ACl)
6 · The paper itself

Abstract

Recent advances in next-generation sequencing and data analysis have provided new gateways for identification of novel genome-wide genetic determinants governing tissue development and disease. These advances have revolutionized our understanding of cellular differentiation, homeostasis, and specialized function in multiple tissues. Bioinformatic and functional analysis of these genetic determinants and the pathways they regulate have provided a novel basis for the design of functional experiments to answer a wide range of long-sought biological questions. A well-characterized model for the application of these emerging technologies is the development and differentiation of the ocular lens and how individual pathways regulate lens morphogenesis, gene expression, transparency, and refraction. Recent applications of next-generation sequencing analysis on well-characterized chicken and mouse lens differentiation models using a variety of omics techniques including RNA-seq, ATAC-seq, whole-genome bisulfite sequencing (WGBS), chip-seq, and CUT&RUN have revealed a wide range of essential biological pathways and chromatin features governing lens structure and function. Multiomics integration of these data has established new gene functions and cellular processes essential for lens formation, homeostasis, and transparency including the identification of novel transcription control pathways, autophagy remodeling pathways, and signal transduction pathways, among others. This review summarizes recent omics technologies applied to the lens, methods for integrating multiomics data, and how these recent technologies have advanced our understanding ocular biology and function. The approach and analysis are relevant to identifying the features and functional requirements of more complex tissues and disease states.

Indexed as

Lens, CrystallineMultiomicsAnimalsChromatinGene Expression RegulationGenomeMiceChromatinATAC-seqbisulfate sequencingchromatinCUT&RUNdevelopmentdifferentiationgene regulationmultiomicsRNA-seq

Identifiers

PMID37189439
PMCPMC10136076

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.