Evidence map›Paper›PMID 37196698›Full record

ReviewAdvanced drug delivery reviews2023

Air-Liquid interface cultures to model drug delivery through the mucociliary epithelial barrier.

Rhianna E Lee, Boris Reidel, Mark R Nelson, Jade K Macdonald, Mehmet Kesimer, Scott H Randell

Open access · greenAbstract readReview
In one paragraph

Review in Advanced drug delivery reviews, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
13.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 51 citations in OpenAlex.

  1. Article
  2. Review
  3. Preclinical human models of primary ciliary dyskinesia.European respiratory review : an official journal of the European Respiratory Society · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Rhianna E LeeMarsico Lung Institute and Cystic Fibrosis Research Center, United States; Department of Cell Biology and Physiology, United States.
Boris ReidelMarsico Lung Institute and Cystic Fibrosis Research Center, United States; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.
Mark R NelsonMarsico Lung Institute and Cystic Fibrosis Research Center, United States.
Jade K MacdonaldMarsico Lung Institute and Cystic Fibrosis Research Center, United States.
Mehmet KesimerMarsico Lung Institute and Cystic Fibrosis Research Center, United States; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.
Scott H RandellMarsico Lung Institute and Cystic Fibrosis Research Center, United States; Department of Cell Biology and Physiology, United States. Electronic address: scott_randell@med.unc.edu.
Cystic Fibrosis Foundation · USLung Institute · USUniversity of North Carolina at Chapel Hill · US

Funding

Vector CoreP30DK065988 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Richard Charles Boucher, Martina Gentzsch · 2004 to 2026
$26.5M
Training Program in Cellular Systems and Integrative PhysiologyT32GM133364 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Kathleen M Caron, Natasha T Snider · 2020 to 2026
$1.4M
Engineering Human Bronchial Epithelial Cells for Cystic Fibrosis Cell TherapyF31HL158197 · NHLBI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI LEE, RHIANNA E · 2021 to 2022
$67k
NHLBI NIH HHS F31 HL158197NIDDK NIH HHS P30 DK065988NIGMS NIH HHS T32 GM133364
6 · The paper itself

Abstract

Epithelial cells from mucociliary portions of the airways can be readily grown and expanded in vitro. When grown on a porous membrane at an air-liquid interface (ALI) the cells form a confluent, electrically resistive barrier separating the apical and basolateral compartments. ALI cultures replicate key morphological, molecular and functional features of the in vivo epithelium, including mucus secretion and mucociliary transport. Apical secretions contain secreted gel-forming mucins, shed cell-associated tethered mucins, and hundreds of additional molecules involved in host defense and homeostasis. The respiratory epithelial cell ALI model is a time-proven workhorse that has been employed in various studies elucidating the structure and function of the mucociliary apparatus and disease pathogenesis. It serves as a critical milestone test for small molecule and genetic therapies targeting airway diseases. To fully exploit the potential of this important tool, numerous technical variables must be thoughtfully considered and carefully executed.

Indexed as

Epithelial CellsMucinsCells, CulturedEpitheliumHumansMucociliary ClearanceMucins

Identifiers

PMID37196698
PMCPMC10336980
OpenAlexW4376620090

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.