ReviewNature protocols2023
Encapsulating and stabilizing enzymes using hydrogen-bonded organic frameworks.
Review in Nature protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 107 citations in OpenAlex.
- Catalytic profiling of extracellular vesicles maps altered cancer metabolism.Nature nanotechnology · 2026Article
- Enhancing Enzyme Activity Via Noncovalent Association with DNA-Functionalized Nanoparticles.JACS Au · 2026Article
- A Cholesterol Analogue for Cell-Surface Enzyme Display.Angewandte Chemie (International ed. in English) · 2026Article
- Hydrophilic-Stable Nucleoside-Based Hydrogen-Bonded Organic Frameworks (N-HOF) for Therapeutic Bacterial Hybrid Systems.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Regulation of Microenvironments of Hydrogen-Bonded Organic Frameworks for Enhanced Enzyme Activity of Phosphotriesterase.Molecules (Basel, Switzerland) · 2026Article
- An orally administrable hydrogen-bonded organic framework (HOF)-based nanoreactor to reprogram metabolism for senescence intervention.National science review · 2026Article
- Reticular Chemistry: A Versatile Platform for Engineering Heterogenous Biocatalysts.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- A Stable Tetraphenylethylene-Based Charge-Assisted Hydrogen-Bonded Organic Framework for Turn-On Fluorescence Sensing of AlMolecules (Basel, Switzerland) · 2025Article
- Enzyme Immobilization in Porous Crystals via Cage Encapsulation.Angewandte Chemie (International ed. in English) · 2025Article
- Enhanced enzyme stability at the interphase of water-oil for continuous-flow olefin epoxidation.Nature communications · 2025Article
- Homologous heteropolyaromatic covalent organic frameworks for enhancing photocatalytic hydrogen peroxide production and aerobic oxidation.Nature communications · 2025Article
- Design, Synthesis, and Application of Immobilized Enzymes on Artificial Porous Materials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Hydrogen-bonded organic frameworks for photocatalytic synthesis of hydrogen peroxide.Nature communications · 2025Article
- Surface-Engineered Nanoparticles Enhance the Peroxidase Activity of Heme-Containing Proteins.ACS nano · 2025Article
- Magnetically Responsive Enzyme and Hydrogen-Bonded Organic Framework Biocomposites for Biosensing.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Boosting the photocatalytic decontamination efficiency using a supramolecular photoenzyme ensemble.Science advances · 2024Article
- Pros and Cons in Various Immobilization Techniques and Carriers for Enzymes.Applied biochemistry and biotechnology · 2024Review
- A Solid-State Transformation of Hydrogen-Bonded Organic Frameworks for Efficient Acetylene Storage and Gas Separation.Chem & bio engineering · 2024Article
- Structural Design and Energy and Environmental Applications of Hydrogen-Bonded Organic Frameworks: A Systematic Review.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Supramolecular polynuclear clusters sustained cubic hydrogen bonded frameworks with octahedral cages for reversible photochromism.Nature communications · 2024Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzymes are outstanding natural catalysts with exquisite 3D structures, initiating countless life-sustaining biotransformations in living systems. The flexible structure of an enzyme, however, is highly susceptible to non-physiological environments, which greatly limits its large-scale industrial applications. Seeking suitable supports to immobilize fragile enzymes is one of the most efficient routes to ameliorate the stability problem. This protocol imparts a new bottom-up strategy for enzyme encapsulation using a hydrogen-bonded organic framework (HOF-101). In short, the surface residues of the enzyme can trigger the nucleation of HOF-101 around its surface through the hydrogen-bonded biointerface. As a result, a series of enzymes with different surface chemistries are able to be encapsulated within a highly crystalline HOF-101 scaffold, which has long-range ordered mesochannels. The details of experimental procedures are described in this protocol, which involve the encapsulating method, characterizations of materials and biocatalytic performance tests. Compared with other immobilization methods, this enzyme-triggering HOF-101 encapsulation is easy to operate and affords higher loading efficiency. The formed HOF-101 scaffold has an unambiguous structure and well-arranged mesochannels, favoring mass transfer and understanding of the biocatalytic process. It takes ~13.5 h for successful synthesis of enzyme-encapsulated HOF-101, 3-4 d for characterizations of materials and ~4 h for the biocatalytic performance tests. In addition, no specific expertise is necessary for the preparation of this biocomposite, although the high-resolution imaging requires a low-electron-dose microscope technology. This protocol can provide a useful methodology to efficiently encapsulate enzymes and design biocatalytic HOF materials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.