Evidence map›Paper›PMID 37205369›Full record

ArticlebioRxiv : the preprint server for biology2023

High performance anion exchange chromatography purification of probiotic bacterial extracellular vesicles enhances purity and anti-inflammatory efficacy.

Nicholas H Piroli, Laura Samantha C Reus, Zuzanna Mamczarz, Sulayman Khan, William E Bentley, Steven M Jay

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Nicholas H PiroliFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Laura Samantha C ReusFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Zuzanna MamczarzFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Sulayman KhanFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
William E BentleyFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
Steven M JayFischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
University of Maryland, College Park · US

Funding

University of Maryland Center of Excellence in Regulatory Science and InnovationU01FD005946 · FDA · UNIV OF MARYLAND, COLLEGE PARK · PI BENTLEY, WILLIAM E., POLLI, JAMES E · 2016 to 2025
$21.5M
Nanohydrocyclones for scalable extracellular vesicle purification and drug loadingR21HL159590 · NHLBI · UNIV OF MARYLAND, COLLEGE PARK · PI DEVOE, DON L, JAY, STEVEN MICHAEL · 2021 to 2022
$421k
FDA HHS U01 FD005946NHLBI NIH HHS R21 HL159590
6 · The paper itself

Abstract

Bacterial extracellular vesicles (BEVs), including outer membrane vesicles (OMVs), have emerged as a promising new class of vaccines and therapeutics to treat cancer and inflammatory diseases, among other applications. However, clinical translation of BEVs is hindered by a current lack of scalable and efficient purification methods. Here, we address downstream BEV biomanufacturing limitations by developing a method for orthogonal size- and charge-based BEV enrichment using tangential flow filtration (TFF) in tandem with high performance anion exchange chromatography (HPAEC). The data show that size-based separation co-isolated protein contaminants, whereas size-based TFF with charged-based HPAEC dramatically improved purity of BEVs produced by probiotic Gram-negative

Identifiers

PMID37205369
PMCPMC10187247
OpenAlexW4367679308

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.