Evidence map›Paper›PMID 37220701›Full record

ArticleJournal of pharmaceutical and biomedical analysis2023

Identification of glycolytic proteins as binding partners of Bri2 BRICHOS domain.

Helene Tigro, Makoto Shimozawa, Per Nilsson, Alexey Lyashkov, Mohammed Khadeer, Ivar Järving, Luigi Ferrucci, Ruth Shimmo, Jan Johansson, Ruin Moaddel

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pharmaceutical and biomedical analysis, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Investigating ITM2B-associated ataxia in a Taiwanese cerebellar ataxia cohort.Annals of clinical and translational neurology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Helene TigroSchool of Natural Sciences and Health, Tallinn University, Tallinn, Estonia; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
Makoto ShimozawaDepartment of Neurobiology, Care Sciences and Society, Division of Neurogeriatrics, Center for Alzheimer Research, Karolinska Institutet, Solna, Sweden.
Per NilssonDepartment of Neurobiology, Care Sciences and Society, Division of Neurogeriatrics, Center for Alzheimer Research, Karolinska Institutet, Solna, Sweden.
Alexey LyashkovBiomedical Research Centre, National Institute on Aging, NIH, Baltimore, MD 21224, United States.
Mohammed KhadeerBiomedical Research Centre, National Institute on Aging, NIH, Baltimore, MD 21224, United States.
Ivar JärvingDepartment of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.
Luigi FerrucciBiomedical Research Centre, National Institute on Aging, NIH, Baltimore, MD 21224, United States.
Ruth ShimmoSchool of Natural Sciences and Health, Tallinn University, Tallinn, Estonia.
Jan JohanssonDepartment of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
Ruin MoaddelBiomedical Research Centre, National Institute on Aging, NIH, Baltimore, MD 21224, United States. Electronic address: moaddelru@mail.nih.gov.
Karolinska Institutet · SENational Institute on Aging · USTallinn University · EETallinn University of Technology · EE

Funding

Immobilized Receptors In Drug DiscoveryZIAAG000295 · NIA · NATIONAL INSTITUTE ON AGING · PI EGAN, JOSEPHINE · 2009 to 2025
$11.4M
Intramural NIH HHS Z99 AG999999Intramural NIH HHS ZIA AG000295
6 · The paper itself

Abstract

Human integral membrane protein 2B (ITM2B or Bri2) is a member of the BRICHOS family, that can attenuate Aβ pathology in the brain. As a result, the identification of novel Bri2 BRICHOS client proteins has been sought to help elucidate signaling pathways and the potential identification of novel therapeutic targets. To identify Bri2 BRICHOS interacting partners, we carried out a 'protein fishing' experiment using recombinant human (rh) Bri2 BRICHOS-coated magnetic particles, in combination with proteomic analysis on cytosolic and membrane fractions of cortical homogenates from C57BL/6 J WT mouse. We identified 4 proteins from the cytosolic fractions and 44 proteins from the membrane fractions that had significant interactions (p < 0.05) with Bri2 BRICHOS domain, of which 11 proteins were previously identified as proteins that interacted with Bri2 BRICHOS domain. Enrichment analysis of the retained proteins identified glycolysis/gluconeogenesis as the most enriched pathway, with several proteins identified playing roles in carbon metabolism, amino acid synthesis. The data suggested that Bri2 BRICHOS may have a role in cellular energy demands in the brain via glycolysis and mitochondrial oxidative phosphorylation and may play a role in mitochondrial homeostasis.

Indexed as

Adaptor Proteins, Signal TransducingProtein BindingAnimalsHumansMiceMice, Inbred C57BLProteomicsAdaptor Proteins, Signal TransducingITM2B protein, humanProtein fishingProtein-protein interactionsProteomicsString analysis

Identifiers

PMID37220701
PMCPMC12036743
OpenAlexW4377030025

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.