Evidence mapPaperPMID 37224335Full record

ArticleDiabetes2023

GDF15 Mediates the Effect of Skeletal Muscle Contraction on Glucose-Stimulated Insulin Secretion.

Hui Zhang, Anny Mulya, Stephan Nieuwoudt, Bolormaa Vandanmagsar, Ruth McDowell, Elizabeth C Heintz, Elizabeth R M Zunica, J Jason Collier, Nadejda Bozadjieva-Kramer, Randy J Seeley and 2 more

Abstract read
In one paragraph

Article in Diabetes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. Trial
  2. Article
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  5. Observational
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  7. Article
  8. Review
  9. Review
  10. GDF15: An emerging disease target and biomarker of metabolic diseases.Journal of endocrinological investigation · 2025
    Review
  11. Article
  12. Review
  13. Article
  14. Review
  15. Review
  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Hui ZhangDepartment of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH.
Anny MulyaDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH.
Stephan NieuwoudtDepartment of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH.
Bolormaa VandanmagsarIntegrated Physiology and Molecular Medicine Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA.
Ruth McDowellDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH.
Elizabeth C HeintzIntegrated Physiology and Molecular Medicine Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA.
Elizabeth R M ZunicaIntegrated Physiology and Molecular Medicine Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA.
J Jason CollierIslet Biology and Inflammation Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA.ORCID 0000-0003-2817-4152
Nadejda Bozadjieva-KramerDepartment of Surgery, University of Michigan, Ann Arbor, MI.
Randy J SeeleyDepartment of Surgery, University of Michigan, Ann Arbor, MI.
Christopher L AxelrodDepartment of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH.
John P KirwanDepartment of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH.ORCID 0000-0001-7321-9917

Funding

Clinical and Translational Science Collaborative of Northern Ohio, Catalyzing Linkages for Everyone's Health (CLE Health)UM1TR004528 · CASE WESTERN RESERVE UNIVERSITY · 2025 to 2025
$7.9M
The role of maternal obesity-driven inflammation and adverse pregnancy outcomes in a mouse model of preeclampsiaP20GM135002 · NIGMS · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · 2022 to 2025
$6.5M
Louisiana Clinical and Translational Science CenterU54GM104940 · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · 2025 to 2025
$3.9M
Pilot and Feasibility ProgramP30DK072476 · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · 2005 to 2025
$2.2M
Age, Exercise, Diet: Effects on Insulin ResistanceR01AG012834 · CASE WESTERN RESERVE UNIVERSITY · 2004 to 2005
$781k
Role and Regulation of Skeletal Muscle Mitochondrial Dynamics in Type 2 DiabetesR01DK108089 · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · 2025 to 2025
$750k
Role of GDF15 and its receptor in the CNS regulation of food intake and body weightR01DK119188 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI RANDY J SEELEY · 2022 to 2022
$466k
AGE, EXERCISE, DIET--EFFECTS ON GLUCOSE/FATTY ACID CYCLER29AG012834 · PENNSYLVANIA STATE UNIVERSITY-UNIV PARK · 1995 to 1999
The role of intestinal-derived FGF15/19 during obesity and rapid weight lossIK2BX005715 · VA · VETERANS HEALTH ADMINISTRATION · 2024 to 2025
BLRD VA IK2 BX005715NCATS NIH HHS UL1 TR002548NCATS NIH HHS UM1 TR004528NCCIH NIH HHS T32 AT004094NCRR NIH HHS UL1 RR024989NIAMS NIH HHS AR067477NIAMS NIH HHS R21 AR067477NIA NIH HHS AG12834NIA NIH HHS R01 AG012834NIA NIH HHS R29 AG012834NIDDK NIH HHS DK108089NIDDK NIH HHS P30 DK072476NIDDK NIH HHS R01 DK108089NIDDK NIH HHS R01 DK119188NIGMS NIH HHS GM104940NIGMS NIH HHS P20 GM135002NIGMS NIH HHS P30 GM118430NIGMS NIH HHS U54 GM104940
6 · The paper itself

Abstract

Exercise is a first-line treatment for type 2 diabetes and preserves β-cell function by hitherto unknown mechanisms. We postulated that proteins from contracting skeletal muscle may act as cellular signals to regulate pancreatic β-cell function. We used electric pulse stimulation (EPS) to induce contraction in C2C12 myotubes and found that treatment of β-cells with EPS-conditioned medium enhanced glucose-stimulated insulin secretion (GSIS). Transcriptomics and subsequent targeted validation revealed growth differentiation factor 15 (GDF15) as a central component of the skeletal muscle secretome. Exposure to recombinant GDF15 enhanced GSIS in cells, islets, and mice. GDF15 enhanced GSIS by upregulating the insulin secretion pathway in β-cells, which was abrogated in the presence of a GDF15 neutralizing antibody. The effect of GDF15 on GSIS was also observed in islets from GFRAL-deficient mice. Circulating GDF15 was incrementally elevated in patients with pre- and type 2 diabetes and positively associated with C-peptide in humans with overweight or obesity. Six weeks of high-intensity exercise training increased circulating GDF15 concentrations, which positively correlated with improvements in β-cell function in patients with type 2 diabetes. Taken together, GDF15 can function as a contraction-induced protein that enhances GSIS through activating the canonical signaling pathway in a GFRAL-independent manner. ARTICLE HIGHLIGHTS: Exercise improves glucose-stimulated insulin secretion through direct interorgan communication. Contracting skeletal muscle releases growth differentiation factor 15 (GDF15), which is required to synergistically enhance glucose-stimulated insulin secretion. GDF15 enhances glucose-stimulated insulin secretion by activating the canonical insulin release pathway. Increased levels of circulating GDF15 after exercise training are related to improvements in β-cell function in patients with type 2 diabetes.

Indexed as

Diabetes Mellitus, Type 2Insulin-Secreting CellsAnimalsGlucoseGrowth Differentiation Factor 15HumansInsulinInsulin SecretionMiceMuscle ContractionMuscle, SkeletalGDF15 protein, humanGlucoseGrowth Differentiation Factor 15Insulin

Identifiers

PMID37224335
PMCPMC10382648

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.