Evidence map›Paper›PMID 37227474›Full record

ArticleApplied microbiology and biotechnology2023

Leptospiral imelysin (LIC_10713) is secretory, immunogenic and binds to laminin, fibronectin, and collagen IV.

Abhijit Sarma, Dhandapani Gunasekaran, Homen Phukan, Akhil Baby, Suneetha Hariharan, Arun Kumar De, Debasis Bhattacharya, Sankar Natesan, Jebasingh Tennyson, Madathiparambil Gopalakrishnan Madanan

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Abhijit SarmaDepartment of Biochemistry, ICMR - Regional Medical Research Centre, Port Blair, 744103, Andaman and Nicobar Islands, India.ORCID http://orcid.org/0000-0002-6093-9760
Dhandapani GunasekaranDepartment of Biochemistry, ICMR - Regional Medical Research Centre, Port Blair, 744103, Andaman and Nicobar Islands, India.ORCID http://orcid.org/0000-0002-1402-3620
Homen PhukanDepartment of Biochemistry, ICMR - Regional Medical Research Centre, Port Blair, 744103, Andaman and Nicobar Islands, India.ORCID http://orcid.org/0000-0002-8781-8870
Akhil BabyDepartment of Genetic Engineering, School of Biotechnology, Madurai Kamraj University, Madurai, Tamil Nadu, 625021, India.ORCID http://orcid.org/0000-0001-5846-624X
Suneetha HariharanDepartment of Biochemistry, ICMR - Regional Medical Research Centre, Port Blair, 744103, Andaman and Nicobar Islands, India.ORCID http://orcid.org/0000-0001-8094-405X
Arun Kumar DeDivision of Animal Science, ICAR- Central Island Agricultural Research Institute, Port Blair, 744105, Andaman and Nicobar Islands, India.ORCID http://orcid.org/0000-0003-0083-9300
Debasis BhattacharyaDivision of Animal Science, ICAR- Central Island Agricultural Research Institute, Port Blair, 744105, Andaman and Nicobar Islands, India.ORCID http://orcid.org/0000-0003-3680-7327
Sankar NatesanDepartment of Genetic Engineering, School of Biotechnology, Madurai Kamraj University, Madurai, Tamil Nadu, 625021, India.ORCID http://orcid.org/0000-0003-0927-8853
Jebasingh TennysonDepartment of Plant Sciences, School of Biological Sciences, Madurai Kamraj University, Madurai, Tamil Nadu, 625021, India.ORCID http://orcid.org/0000-0001-5771-2189
Madathiparambil Gopalakrishnan MadananDepartment of Biochemistry, ICMR - Regional Medical Research Centre, Port Blair, 744103, Andaman and Nicobar Islands, India. madanan.mg@icmr.gov.in.ORCID http://orcid.org/0000-0002-5119-0081

Funding

Department of Science and Technology, Science and Engineering Research Board, New Delhi, India SR/SO/HS/0108/2012Indian Council of Medical Research, New Delhi, India. Grant No: Fellowship/131/2022-ECD-II-2021-8230Indian Council of Medical Research, New Delhi, India. ICMR-SRF 2020-0756/PROTEOMICS-BMSIndian Council of Medical Research, New Delhi, India. Leptos/22/2013-ECD-I-2012-2400
6 · The paper itself

Abstract

Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira. Early and accurate diagnosis is the prime step in managing the disease. Secretory proteins of Leptospira remain distinguished for diagnosis due to their availability as soluble proteins in the serum and their interaction with the host immune response due to their extracellular presence. This study presents the cloning, expression, purification, and characterization of imelysin or LruB (LIC_10713), a putative leptospiral protein. We report that the localization of imelysin showed its presence in the inner membrane and in the culture supernatant. The imelysin was upregulated under in vitro physiological conditions of infection. The LIC_10713 interacted significantly with laminin, fibronectin, collagen type I, and collagen type IV in a dose-dependent manner. Phylogenetic analysis showed that LIC_10713 is predominately found in the pathogenic species of Leptospira, and the GxHxxE motif of imelysin-like proteins is represented as the amino acid sequence G

Indexed as

LeptospiraLeptospira interrogansLeptospirosisCollagen Type IVFibronectinsHumansLamininPhylogenyProtein BindingCollagen Type IVFibronectinsLamininDiagnosisImelysinInvasinLeptospiraLeptospirosisLIC10713Peptidase M75Putative lipoproteinSecretory

Identifiers

PMID37227474

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.