Evidence map›Paper›PMID 37233978›Full record

ArticleJournal of endocrinological investigation2023

Protein kinase C delta mediates Pasireotide effects in an ACTH-secreting pituitary tumor cell line.

E Gentilin, P Borges De Souza, M R Ambrosio, M Bondanelli, I Gagliardi, M C Zatelli

Open access · hybridAbstract read
In one paragraph

Article in Journal of endocrinological investigation, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 86% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

E GentilinBioacoustics Research Laboratory, Department of Neurosciences, University of Padua, Padua, Italy. erica.gentilin@unipd.it.
P Borges De SouzaSection of Endocrinology, Geriatrics & Internal Medicine, Dept. of Medical Sciences, University of Ferrara, Ferrara, Italy.
M R AmbrosioSection of Endocrinology, Geriatrics & Internal Medicine, Dept. of Medical Sciences, University of Ferrara, Ferrara, Italy.
M BondanelliSection of Endocrinology, Geriatrics & Internal Medicine, Dept. of Medical Sciences, University of Ferrara, Ferrara, Italy.
I GagliardiSection of Endocrinology, Geriatrics & Internal Medicine, Dept. of Medical Sciences, University of Ferrara, Ferrara, Italy.
M C ZatelliSection of Endocrinology, Geriatrics & Internal Medicine, Dept. of Medical Sciences, University of Ferrara, Ferrara, Italy. ztlmch@unife.it.ORCID http://orcid.org/0000-0001-8408-7796
University of Ferrara · ITUniversity of Padua · IT

Funding

Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Ferrara FAR 2021MIUR PRIN 2017Z3N3YC
6 · The paper itself

Abstract

purposeClinical control of corticotroph tumors is difficult to achieve since they usually persist or relapse after surgery. Pasireotide is approved to treat patients with Cushing's disease for whom surgical therapy is not an option. However, Pasireotide seems to be effective only in a sub-set of patients, highlighting the importance to find a response marker to this approach. Recent studies demonstrated that the delta isoform of protein kinase C (PRKCD) controls viability and cell cycle progression of an in vitro model of ACTH-secreting pituitary tumor, the AtT-20/D16v-F2 cells. This study aims at exploring the possible PRKCD role in mediating Pasireotide effects.

methodsIt was assessed cell viability, POMC expression and ACTH secretion in AtT20/D16v-F2 cells over- or under-expressing PRKCD.

resultsWe found that Pasireotide significantly reduces AtT20/D16v-F2 cell viability, POMC expression and ACTH secretion. In addition, Pasireotide reduces miR-26a expression. PRKCD silencing decreases AtT20/D16v-F2 cell sensitivity to Pasireotide treatment; on the contrary, PRKCD overexpression increases the inhibitory effects of Pasireotide on cell viability and ACTH secretion.

conclusionOur results provide new insights into potential PRKCD contribution in Pasireotide mechanism of action and suggest that PRKCD might be a possible marker of therapeutic response in ACTH-secreting pituitary tumors.

Indexed as

Pituitary ACTH HypersecretionPituitary NeoplasmsAdrenocorticotropic HormoneCell LineCell Line, TumorCorticotrophsHumansNeoplasm Recurrence, LocalPro-OpiomelanocortinProtein Kinase C-deltaSomatostatinAdrenocorticotropic HormonepasireotidePro-OpiomelanocortinProtein Kinase C-deltaSomatostatinACTHCorticotroph tumorsPasireotidePOMCPRKCD

Identifiers

PMID37233978
PMCPMC10632222
OpenAlexW4378349069

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.