Evidence map›Paper›PMID 37236490›Full record

ArticleGeneral and comparative endocrinology2023

The nuclear localization sequence and C-terminus of parathyroid hormone-related protein regulate normal pancreatic islet development and function.

Ibiagbani M Max-Harry, Waleed J Hashmi, Brian P List, Noriko Kantake, Kathryn L Corbin, Ramiro E Toribio, Craig S Nunemaker, Thomas J Rosol

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in General and comparative endocrinology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Ibiagbani M Max-HarryDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA; Diabetes Institute, Heritage College of Osteopathic Medicine, Ohio University, USA; Molecular and Cellular Biology Program, College of Arts and Sciences, Ohio University, USA; Department of Biological Sciences, Ohio University, USA.
Waleed J HashmiDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA; Molecular and Cellular Biology Program, College of Arts and Sciences, Ohio University, USA.
Brian P ListDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA; Diabetes Institute, Heritage College of Osteopathic Medicine, Ohio University, USA; Translational Biomedical Sciences Program, Graduate College, Ohio University, USA.
Noriko KantakeDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA.
Kathryn L CorbinDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA.
Ramiro E ToribioDepartment of Veterinary Clinical Sciences, The Ohio State University, Columbus, OH, USA.
Craig S NunemakerDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA; Diabetes Institute, Heritage College of Osteopathic Medicine, Ohio University, USA; Translational Biomedical Sciences Program, Graduate College, Ohio University, USA. Electronic address: nunemake@ohio.edu.
Thomas J RosolDepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45701, USA; Ohio Musculoskeletal and Neurological Institute (OMNI), Heritage College of Osteopathic Medicine, Ohio University, USA. Electronic address: rosolt@ohio.edu.
Ohio University · USFlorida State University · USThe Ohio State University · US

Funding

PTHrP on normal osteoblast differentiation and functionK01RR018924 · NCRR · OHIO STATE UNIVERSITY · PI TORIBIO, RAMIRO ELISEO · 2003 to 2008
$630k
Small Laboratory Animal Imaging: High Resolution Microcomputed Tomography SystemS10RR020888 · NCRR · OHIO STATE UNIVERSITY · PI TORIBIO, RAMIRO ELISEO · 2006 to 2006
$458k
NCRR NIH HHS K01 RR018924NCRR NIH HHS S10 RR020888
6 · The paper itself

Abstract

Parathyroid hormone-related protein (PTHrP) is a pleiotropic hormone essential for morphogenesis, tissue differentiation, as well as cell regulation and function. PTHrP is expressed by pancreatic beta cells which are responsible for insulin secretion. Previous studies have reported that N-terminal PTHrP stimulated proliferation in beta cells in rodents. We have developed a knockin mouse model (PTHrP Δ/Δ) lacking the C-terminal and nuclear localization sequence (NLS) of PTHrP. These mice die at ∼day 5, are severely stunted in growth, weigh 54% less than control mice at day 1-2 and eventually fail to grow. PTHrP Δ/Δ mice are also hypoinsulinemic and hypoglycemic yet have nutrient intake proportional to size. To characterize the pancreatic islets in these mice, islets (∼10-20) were isolated from 2 to 5 day-old-mice using collagenase digestion. Islets from PTHrP Δ/Δ mice were smaller in size but secreted more insulin than littermate controls. PTHrP Δ/Δ and control mice islets were exposed to various glucose concentrations and intracellular calcium, the trigger for insulin release, was elevated for glucose concentrations of 8-20 mM. Immunofluorescence staining showed less glucagon-stained area in islets from PTHrP Δ/Δ mice (∼250 µm

Indexed as

Islets of LangerhansParathyroid Hormone-Related ProteinAnimalsGlucagonGlucoseInsulinMiceGlucagonGlucoseInsulinParathyroid Hormone-Related ProteinCalciumGlucagonGlucoseInsulinIsletIslet of LangerhansPancreasPTHrP

Identifiers

PMID37236490
PMCPMC10323322
OpenAlexW4377969262

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.