Evidence map›Paper›PMID 37238034›Full record

ArticleAnimals : an open access journal from MDPI2023

The In Vitro Evaluation of Rooster Semen Pellets Frozen with Dimethylacetamide.

Shaimaa K Hamad, Ahmed M Elomda, Yanyan Sun, Yunlei Li, Yunhe Zong, Jilan Chen, Ahmed O Abbas, Farid K R Stino, Ali Nazmi, Gamal M K Mehaisen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Shaimaa K HamadDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Ahmed M ElomdaDepartment of Animal Biotechnology, Animal Production Research Institute, Agriculture Research Center, Giza 12613, Egypt.ORCID 0000-0001-6115-8408
Yanyan SunKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.ORCID 0000-0001-6425-7786
Yunlei LiKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Yunhe ZongKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Jilan ChenKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Ahmed O AbbasDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Farid K R StinoDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
Ali NazmiDepartment of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
Gamal M K MehaisenDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.ORCID 0000-0003-2006-4290

Funding

The Egyptian Academy of Scientific Research and Technology CHINA5105The Egyptian Ministry of Higher Education, Cairo University, and Ohio State University S.K.H. PhD grantThe National Natural Science Foundation of China 31961143028
6 · The paper itself

Abstract

Sperm cryopreservation is an effective technique for conserving animal genetic diversity and transmitting superior genetic backgrounds, maintained via a non-invasive sampling and collection of huge quantities of sperm. Nevertheless, cryopreservation in avian species is not commercially viable because of the rooster sperm's susceptibility to damage. This study aims to estimate the impact of dimethylacetamide (DMA) as a cryoprotectant at different levels (3%, 6%, or 9%) on the post-thawed sperm quality, motility, antioxidant-biomarkers, and the expression of anti-freeze related genes. Semen samples were collected twice a week from twelve roosters aged 40 wk, weighing 3400 ± 70 g, and belonging to the Cairo-B2 chicken strain. Fresh semen samples were rapidly appraised, pooled, diluted with two volumes of a basic extender, and divided equally into three groups. The diluted groups were chilled at -20 °C for 7 min, then gently supplemented with 3, 6, or 9% pre-cooled DMA and equilibrated at 5 °C for a further 10 min. Semen pellets were formed by pipetting drops 7 cm above liquid nitrogen (LN

Indexed as

cryopreservationdimethylacetamideroosterssemensperm motility

Identifiers

PMID37238034
PMCPMC10215955

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.