ArticlePlant physiology2023
Allelic variation in transcription factor PtoWRKY68 contributes to drought tolerance in Populus.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 35 citations in OpenAlex.
- Abscisic Acid-Mediated Drought Tolerance inCurrent issues in molecular biology · 2026Review
- Natural variation inScience advances · 2026Article
- Genome-Wide Association Study Reveals the Genetic Architecture and Key Drought and Yield Related Genes in Common Vetch (Vicia sativa L.).Plant biotechnology journal · 2026Article
- Identification and characterization of the DREB family in Chinese yam and functional study of DoDREB28 under drought stress.BMC plant biology · 2026Article
- Study on the positive regulation of drought tolerance by PdMYB2R032 based on R2R3-MYB gene family analysis in Populus deltoides.BMC plant biology · 2026Article
- Climate and hybridization shape stomatal trait evolution in Populus.The New phytologist · 2026Article
- Integrated transcriptomic and metabolomic analysis of provenances and organ-specific responses to drought and rehydration inFrontiers in plant science · 2026Article
- Transcription-metabolism association analysis of molecular mechanisms in sweet orange plants in response to boron deficiency.BMC genomics · 2025Article
- Natural variants ofScience advances · 2025Article
- Allelic variants of IRKI contribute to photosynthetic efficiency by regulating rubisco activase in Populus.Plant physiology · 2025Article
- CrWRKY57 and CrABF3 cooperatively activateHorticulture research · 2025Article
- Genome-wide association studies in forestry.Molecular biology reports · 2025Review
- Hierarchical Regulatory Networks Reveal Conserved Drivers of Plant Drought Response at the Cell-Type Level.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Integrative physiological and transcriptomic analysis provides insights on the molecular basis of ABA-enhanced drought tolerance in pear (Pyrus betulaefolia).BMC plant biology · 2025Article
- Transcription FactorPlants (Basel, Switzerland) · 2025Article
- The transcription factor MYC2 positively regulates terpene trilactone biosynthesis through activatingHorticulture research · 2024Article
- The MdHSC70-MdWRKY75 module mediates basal apple thermotolerance by regulating the expression of heat shock factor genes.The Plant cell · 2024Article
- Function identification of miR159a, a positive regulator during poplar resistance to drought stress.Horticulture research · 2023Article
- The miRNA-mRNA Regulatory Modules ofInternational journal of molecular sciences · 2023Article
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Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drought stress limits woody species productivity and influences tree distribution. However, dissecting the molecular mechanisms that underpin drought responses in forest trees can be challenging due to trait complexity. Here, using a panel of 300 Chinese white poplar (Populus tomentosa) accessions collected from different geographical climatic regions in China, we performed a genome-wide association study (GWAS) on seven drought-related traits and identified PtoWRKY68 as a candidate gene involved in the response to drought stress. A 12-bp insertion and/or deletion and three nonsynonymous variants in the PtoWRKY68 coding sequence categorized natural populations of P. tomentosa into two haplotype groups, PtoWRKY68hap1 and PtoWRKY68hap2. The allelic variation in these two PtoWRKY68 haplotypes conferred differential transcriptional regulatory activities and binding to the promoters of downstream abscisic acid (ABA) efflux and signaling genes. Overexpression of PtoWRKY68hap1 and PtoWRKY68hap2 in Arabidopsis (Arabidopsis thaliana) ameliorated the drought tolerance of two transgenic lines and increased ABA content by 42.7% and 14.3% compared to wild-type plants, respectively. Notably, PtoWRKY68hap1 (associated with drought tolerance) is ubiquitous in accessions in water-deficient environments, whereas the drought-sensitive allele PtoWRKY68hap2 is widely distributed in well-watered regions, consistent with the trends in local precipitation, suggesting that these alleles correspond to geographical adaptation in Populus. Moreover, quantitative trait loci analysis and an electrophoretic mobility shift assay showed that SHORT VEGETATIVE PHASE (PtoSVP.3) positively regulates the expression of PtoWRKY68 under drought stress. We propose a drought tolerance regulatory module in which PtoWRKY68 modulates ABA signaling and accumulation, providing insight into the genetic basis of drought tolerance in trees. Our findings will facilitate molecular breeding to improve the drought tolerance of forest trees.
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