Evidence map›Paper›PMID 37258763›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue.

Raquel Saborano, Emma Shepherd, Ulrich L Günther, Patricia F Lalor

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 78% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Raquel SaboranoCentre for Liver and Gastroenterology Research, and NIHR Birmingham Biomedical Research Centre, University of Birmingham, Birmingham, UK.
Emma ShepherdSchool of Biosciences, College of Health and Life Sciences, Aston University, Birmingham, UK.
Ulrich L GüntherInstitute for Chemistry and Metabolomics, University of Luebeck, Luebeck, Germany.
Patricia F LalorCentre for Liver and Gastroenterology Research, and NIHR Birmingham Biomedical Research Centre, University of Birmingham, Birmingham, UK. p.f.lalor@bham.ac.uk.
University of Birmingham · GBAston University · GBInstitute for Integrative and Experimental Genomics · DE

Funding

Department of Health
6 · The paper itself

Abstract

Human metabolic liver disease is dramatically increasing globally and presents an urgent clinical unmet need. Rodent models of non-alcoholic fatty liver disease (NAFLD) are available, but they fail to fully recreate the metabolic and cellular features of human disease. Thus, it is imperative to understand the metabolic interplay in human cells in the context of disease. We have applied nuclear magnetic resonance (NMR) spectroscopy approaches to enable the detection of numerous metabolites in human cells and within intact tissue in a single measurement. In this chapter, we describe the challenges of using isolated human hepatocytes vs perfused human liver tissue for metabolic tracer experiments and how experimental parameters can be refined to interrogate signals from intact tissue and cells.

Indexed as

LiverNon-alcoholic Fatty Liver DiseaseHepatocytesHumansMagnetic Resonance ImagingMagnetic Resonance Spectroscopy1D NMR2D NMRHumanLiverPerfusion

Identifiers

PMID37258763
OpenAlexW4378805261

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.