Evidence mapPaperPMID 37259014Full record

ArticleInflammation2023

Interleukin 4 Reduces Brain Hyperexcitability after Traumatic Injury by Downregulating TNF-α, Upregulating IL-10/TGF-β, and Potential Directing Macrophage/Microglia to the M2 Anti-inflammatory Phenotype.

Mozhdeh Radpour, Bahar Khoshkroodian, Tara Asgari, Hamid Gholami Pourbadie, Mohammad Sayyah

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Article in Inflammation, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 27 citations in OpenAlex.

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  18. Interleukins in Epilepsy: Friend or Foe.Neuroscience bulletin · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Mozhdeh RadpourDepartment of Physiology and Pharmacology, Pasteur Institute of Iran , Tehran, Iran.ORCID http://orcid.org/0000-0003-2463-8087
Bahar KhoshkroodianDepartment of Physiology and Pharmacology, Pasteur Institute of Iran , Tehran, Iran.ORCID http://orcid.org/0000-0001-6315-6254
Tara AsgariDepartment of Physiology and Pharmacology, Pasteur Institute of Iran , Tehran, Iran.ORCID http://orcid.org/0000-0003-1668-6820
Hamid Gholami PourbadieDepartment of Physiology and Pharmacology, Pasteur Institute of Iran , Tehran, Iran.ORCID http://orcid.org/0000-0002-7634-7428
Mohammad SayyahDepartment of Physiology and Pharmacology, Pasteur Institute of Iran , Tehran, Iran. sayyahm2@yahoo.com.ORCID http://orcid.org/0000-0003-0603-2444
Pasteur Institute of Iran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Macrophage/microglia are activated after Traumatic brain injury (TBI), transform to inflammatory phenotype (M1) and trigger neuroinflammation, which provokes epileptogenesis. Interleukin-4 (IL-4) is a well-known drive of macrophage/microglia to the anti-inflammatory phenotype (M2). We tested effect of IL-4 on speed of epileptogenesis, brain expression of inflammatory and anti-inflammatory cytokines, and lesion size in TBI-injured male rats. Rats underwent TBI by Controlled Cortical Impact. Then 100 ng IL-4 was injected into cerebral ventricles. One day after TBI, pentylenetetrazole (PTZ) kindling started and development of generalized seizures was recorded. The lesion size, cell survival rate, TNF-α, TGF-β, IL-10, and Arginase1 (Arg1) was measured in the brain 6 h, 12 h, 24 h, 48 h, and 5 days after TBI. Astrocytes and macrophage/microglia activation/polarization was assessed by GFAP/Arg1 and Iba1/Arg1 immunostaining. TBI-injured rats were kindled by 50% less PTZ injections than control and sham-operated rats. IL-4 did not change kindling rate in sham-operated rats but inhibited acceleration of kindling rate in the TBI-injured rats. IL-4 decreased damage volume and number of destroyed neurons. IL-4 stopped TNF-α whereas upregulated TGF-β, IL-10, and Arg1 expressions. Iba1/Arg1 positive macrophage/microglia was notably increased 48 h after IL-4 administration. IL-4 suppresses TBI-induced acceleration of epileptogenesis in rats by directing TBI neuroinflammation toward an anti-inflammatory tone and inhibition of cell death.

Indexed as

Brain Injuries, TraumaticInterleukin-4AnimalsAnti-Inflammatory AgentsBrainInterleukin-10MacrophagesMaleMicrogliaNeuroinflammatory DiseasesPhenotypeRatsTransforming Growth Factor betaTumor Necrosis Factor-alphaAnti-Inflammatory AgentsInterleukin-10Interleukin-4Transforming Growth Factor betaTumor Necrosis Factor-alphaarginase1kindlingrattraumatraumatic epilepsy.

Identifiers

PMID37259014
OpenAlexW4378953841

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.