Normal cell cycle progression requires negative regulation of E2F1 by Groucho during S phase and its relief at G2 phase.

Shaked Bar-Cohen, María Lorena Martínez Quiles, Alexey Baskin, Ruba Dawud, Barbara H Jennings, Ze'ev Paroush

Open access · hybridAbstract read

In one paragraph

Article in Development (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.2field-weighted citation impact, top 42% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Shaked Bar-CohenDepartment of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University, Jerusalem 91120, Israel.

María Lorena Martínez QuilesDepartment of Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK.

Alexey BaskinDepartment of Biological Chemistry, The Institute of Life Science, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Ruba DawudDepartment of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University, Jerusalem 91120, Israel.

Barbara H JenningsDepartment of Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, UK.ORCID 0000-0003-1696-5256

Ze'ev ParoushDepartment of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University, Jerusalem 91120, Israel.ORCID 0000-0002-5467-2836

Hebrew University of Jerusalem · ILOxford Brookes University · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The cell cycle depends on a sequence of steps that are triggered and terminated via the synthesis and degradation of phase-specific transcripts and proteins. Although much is known about how stage-specific transcription is activated, less is understood about how inappropriate gene expression is suppressed. Here, we demonstrate that Groucho, the Drosophila orthologue of TLE1 and other related human transcriptional corepressors, regulates normal cell cycle progression in vivo. We show that, although Groucho is expressed throughout the cell cycle, its activity is selectively inactivated by phosphorylation, except in S phase when it negatively regulates E2F1. Constitutive Groucho activity, as well as its depletion and the consequent derepression of e2f1, cause cell cycle phenotypes. Our results suggest that Cdk1 contributes to phase-specific phosphorylation of Groucho in vivo. We propose that Groucho and its orthologues play a role in the metazoan cell cycle that may explain the links between TLE corepressors and several types of human cancer.

Indexed as

Basic Helix-Loop-Helix ProteinsDrosophila ProteinsE2F1 Transcription FactorRepressor ProteinsAnimalsCell CycleCo-Repressor ProteinsDrosophilaG2 PhaseS PhaseTranscription FactorsBasic Helix-Loop-Helix ProteinsCo-Repressor ProteinsDrosophila ProteinsE2f1 protein, DrosophilaE2F1 Transcription Factorgro protein, DrosophilaRepressor ProteinsTranscription FactorsCell cycle regulationDrosophilaE2F1GrouchoProtein phosphorylationRepression

Identifiers

PMID37260146

DOI10.1242/dev.201041

PMCPMC10281550

OpenAlexW4376630466

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.