ArticleFrontiers in plant science2023
An efficient transformation method for genome editing of elite bread wheat cultivars.
Article in Frontiers in plant science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Wheat's Up with CRISPR-Cas-Current Advances, Obstacles and Perspectives.International journal of molecular sciences · 2026Review
- Molecular regulators of regeneration and strategies for overcoming genotype-dependent recalcitrance in wheat (Triticum aestivum).Communications biology · 2026Review
- TaGα knockout in wheat causes early heading and short organ length, with dose-dependent effects through various pathways.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Impact of wheat GRF4-GIF1 morphogenic regulators on transformation and genome editing efficiency in elite barley cultivars.Frontiers in plant science · 2026Article
- The illusion of progress: why most introgressions from wild Triticeae fail to improve wheat agronomically.Frontiers in plant science · 2026Review
- Fine tuning wheat heading time through genome editing of transcription factor binding sites in Ppd-1 gene promoter.Scientific reports · 2025Article
- A Protocol for High-efficiency Transformation and Genome Editing in Elite Wheat Cultivars.Methods in molecular biology (Clifton, N.J.) · 2025Article
- CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops.Frontiers in genome editing · 2025Review
- Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency.Biotechnology journal · 2024Review
- Review
- Knockout mutation inFrontiers in genome editing · 2024Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An efficient genetic transformation protocol is necessary to edit genes for trait improvement directly in elite bread wheat cultivars. We used a protein fusion between a wheat growth-regulating factor 4 (GRF4) and its interacting factor (GIF1) to develop a reproducible genetic transformation and regeneration protocol, which we then used to successfully transform elite bread wheat cultivars Baj, Kachu, Morocco, Reedling, RL6077, and Sujata in addition to the experimental cultivar Fielder. Immature embryos were transformed with the vector using particle bombardment method. Transformation frequency increased nearly 60-fold with the GRF4-GIF1-containing vectors as compared to the control vector and ranged from ~5% in the cultivar Kachu to 13% in the cultivar RL6077. We then edited two genes that confer resistance against leaf rust and powdery mildew directly in the aforementioned elite cultivars. A wheat promoter, TaU3 or TaU6, to drive the expression of guide RNA was effective in gene editing whereas the OsU3 promoter failed to generate any edits. Editing efficiency was nearly perfect with the wheat promoters. Our protocol has made it possible to edit genes directly in elite wheat cultivars and would be useful for gene editing in other wheat varieties, which have been recalcitrant to transformation thus far.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.