Evidence map›Paper›PMID 37276389›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2023

split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80.

Ben Ewen-Campen, Haojiang Luan, Jun Xu, Rohit Singh, Neha Joshi, Tanuj Thakkar, Bonnie Berger, Benjamin H White, Norbert Perrimon

Open access · hybridAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
5.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 36 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Sleep-dependent clearance of brain lipids by peripheral blood cells.bioRxiv : the preprint server for biology · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. The Power of Drosophila in Modeling Cancer Cachexia.Advances in experimental medicine and biology · 2025
    Review
  12. Article
  13. Review
  14. Hox gene-specific cellular targeting using split intein Trojan exons.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  15. Expanding theeLife · 2024
    Article
  16. Toward building a library of cell type-specific drivers across developmental stages.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  17. Using single-cell RNA sequencing to generate predictive cell-type-specific split-GAL4 reagents throughout development.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  18. Dissecting neuron-specific functions of circadian genes using modified cell-specific CRISPR approaches.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Ben Ewen-CampenDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.ORCID 0000-0002-4326-3033
Haojiang LuanLaboratory of Molecular Biology, National Institute of Mental Health, NIH, Bethesda, MD 20892.
Jun XuDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.
Rohit SinghComputer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139.
Neha JoshiDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.
Tanuj ThakkarDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.
Bonnie BergerComputer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139.
Benjamin H WhiteLaboratory of Molecular Biology, National Institute of Mental Health, NIH, Bethesda, MD 20892.ORCID 0000-0003-0612-8075
Norbert PerrimonDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115.ORCID 0000-0001-7542-472X
Harvard University · USMassachusetts Institute of Technology · USNational Institute of Mental Health · US

Funding

Identifying Neural Substrates of Behavior in Drosophila MelanogasterZIAMH002800 · NIMH · NATIONAL INSTITUTE OF MENTAL HEALTH · PI WHITE, BENJAMIN H · 2009 to 2025
$29.2M
Functional genomics resources for the Drosophila - TR&D3P41GM132087 · NIGMS · HARVARD MEDICAL SCHOOL · PI MOHR, STEPHANIE E · 2019 to 2023
$6.8M
Using CRISPR technology to study the function of paralogous genesR24OD026435 · OD · HARVARD MEDICAL SCHOOL · PI BELLEN, HUGO J, PERRIMON, NORBERT · 2018 to 2021
$2.5M
Resources for functional studies in DrosophilaR24OD031952 · OD · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT · 2022 to 2025
$2.2M
Manifold representations and active learning for 21 st century biologyR35GM141861 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BERGER, BONNIE · 2021 to 2025
$1.9M
Intramural NIH HHS ZIA MH002800NIGMS NIH HHS P41 GM132087NIGMS NIH HHS R35 GM141861NIH HHS R24 OD026435NIH HHS R24 OD031952
6 · The paper itself

Abstract

The split-Gal4 system allows for intersectional genetic labeling of highly specific cell types and tissues in

Indexed as

Drosophila ProteinsTranscription FactorsAnimalsDrosophilaInteinsProtein SplicingTransgenesDrosophila ProteinsGAL4 protein, DrosophilaTranscription FactorsDrosophilaintersectional geneticssingle-cell transcriptomicssplit-Gal4

Identifiers

PMID37276389
PMCPMC10268248
OpenAlexW4379467121

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.