Evidence map›Paper›PMID 37276413›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2023

NgR1 binding to reovirus reveals an unusual bivalent interaction and a new viral attachment protein.

Danica M Sutherland, Michael Strebl, Melanie Koehler, Olivia L Welsh, Xinzhe Yu, Liya Hu, Rita Dos Santos Natividade, Jonathan J Knowlton, Gwen M Taylor, Rodolfo A Moreno and 11 more

Open access · greenAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 5 institutions in 3 countries.

Danica M SutherlandDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.ORCID 0000-0001-9328-4994
Michael StreblInterfaculty Institute of Biochemistry, University of Tübingen, D-72076 Tübingen, Germany.
Melanie KoehlerLouvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.
Olivia L WelshDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.ORCID 0000-0002-8039-7807
Xinzhe YuVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030.
Liya HuVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030.ORCID 0000-0002-8340-8911
Rita Dos Santos NatividadeLouvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.ORCID 0000-0002-0123-4760
Jonathan J KnowltonDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.ORCID 0000-0002-1630-8734
Gwen M TaylorDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.ORCID 0000-0002-9276-087X
Rodolfo A MorenoVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030.
Patrick WörzInterfaculty Institute of Biochemistry, University of Tübingen, D-72076 Tübingen, Germany.
Zachery R LonerganCryo-Electron Microscopy and Tomography Core, Baylor College of Medicine, Houston, TX 77030.ORCID 0000-0001-7694-2166
Pavithra AravamudhanDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.
Camila Guzman-CardozoDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.
Sukhleen KourDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.
Udai Bhan PandeyDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.ORCID 0000-0002-6267-0179
David AlsteensLouvain Institute of Biomolecular Science and Technology, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium.ORCID 0000-0001-9229-113X
Zhao WangVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030.ORCID 0000-0003-4897-9986
B V Venkataram PrasadVerna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030.ORCID 0000-0002-1172-2071
Thilo StehleInterfaculty Institute of Biochemistry, University of Tübingen, D-72076 Tübingen, Germany.
Terence S DermodyDepartment of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA 15224.ORCID 0000-0003-1853-8741
University of Pittsburgh · USBaylor College of Medicine · USUniversity of Tübingen · DEUCLouvain · BEChildren's Hospital of Pittsburgh · US

Funding

MOLECULAR BASIS OF REOVIRUS PATHOGENESISR01AI038296 · NIAID · VANDERBILT UNIVERSITY · PI DERMODY, TERENCE S. · 1996 to 2019
$3.3M
MOLECULAR PARASITOLOGY TRAINING PROGRAMT32AI007281 · NIAID · MEHARRY MEDICAL COLLEGE · PI NDE, PIUS N · 1985 to 2025
$3.2M
Reovirus NeuropathogenesisR01AI174526 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI TERENCE S. DERMODY · 2022 to 2026
$2.7M
Reovirus Attachment MechanismsR01AI118887 · NIAID · VANDERBILT UNIVERSITY MEDICAL CENTER · PI DERMODY, TERENCE S. · 2015 to 2019
$2.3M
Molecular mechanism of Androgen Receptor mediated transcriptionR01GM143380 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI WANG, ZHAO · 2021 to 2025
$1.7M
Investigation of the Cellular and Molecular Mechanisms of Thrombocyte Integrin SignalingR01HL162842 · NHLBI · BAYLOR COLLEGE OF MEDICINE · PI WANG, ZHAO · 2022 to 2025
$1.6M
NHLBI NIH HHS R01 HL162842NIAID NIH HHS R01 AI038296NIAID NIH HHS R01 AI118887NIAID NIH HHS R01 AI174526NIAID NIH HHS T32 AI007281NIGMS NIH HHS R01 GM143380
6 · The paper itself

Abstract

Nogo-66 receptor 1 (NgR1) binds a variety of structurally dissimilar ligands in the adult central nervous system to inhibit axon extension. Disruption of ligand binding to NgR1 and subsequent signaling can improve neuron outgrowth, making NgR1 an important therapeutic target for diverse neurological conditions such as spinal crush injuries and Alzheimer's disease. Human NgR1 serves as a receptor for mammalian orthoreovirus (reovirus), but the mechanism of virus-receptor engagement is unknown. To elucidate how NgR1 mediates cell binding and entry of reovirus, we defined the affinity of interaction between virus and receptor, determined the structure of the virus-receptor complex, and identified residues in the receptor required for virus binding and infection. These studies revealed that central NgR1 surfaces form a bridge between two copies of viral capsid protein σ3, establishing that σ3 serves as a receptor ligand for reovirus. This unusual binding interface produces high-avidity interactions between virus and receptor to prime early entry steps. These studies refine models of reovirus cell-attachment and highlight the evolution of viruses to engage multiple receptors using distinct capsid components.

Indexed as

OrthoreovirusReoviridaeAnimalsHumansLigandsMammalsNogo Receptor 1Receptors, VirusViral ProteinsVirus AttachmentLigandsNogo Receptor 1Receptors, VirusViral ProteinsattachmentNogo receptor 1receptorstructurevirus

Identifiers

PMID37276413
PMCPMC10268256
OpenAlexW4379467360

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.